TY - JOUR
T1 - Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia
AU - Suturina, Larisa
AU - Lizneva, Daria
AU - Atalyan, Alina
AU - Lazareva, Ludmila
AU - Belskikh, Aleksey
AU - Bairova, Tatyana
AU - Sholokhov, Leonid
AU - Rashidova, Maria
AU - Danusevich, Irina
AU - Nadeliaeva, Iana
AU - Belenkaya, Lilia
AU - Darzhaev, Zorikto
AU - Sharifulin, Eldar
AU - Belkova, Natalia
AU - Igumnov, Ilia
AU - Trofimova, Tatyana
AU - Khomyakova, Anastasiya
AU - Ievleva, Kseniia
AU - Babaeva, Natalia
AU - Egorova, Irina
AU - Salimova, Madinabonu
AU - Yildiz, Bulent O.
AU - Legro, Richard S.
AU - Stanczyk, Frank Z.
AU - Azziz, Ricardo
N1 - Funding Information:
This project was supported by the budget of the state scientific programs: “The main determinants and mechanisms of the formation of reproductive disorders of the family’s reproductive health in various gender and age groups” (#AAAA-A19-119101590007-8), “Early detection and correction of neuro-endocrine, metabolic and psycho-emotional manifestations of reproductive disorders associated with hyperandrogenism” (#AAAA-A18-118011990043-5), and “Pathophysiological mechanisms, genetic and metabolic predictors of reproductive health and longevity in various age, gender and ethnic groups” (#121022500180-6). The research was performed using the equipment of the Center for the Development of Progressive Personalized Health Technologies at the Scientific Center for Family Health and Human Reproduction.
Publisher Copyright:
© 2022 by the authors.
PY - 2023/1
Y1 - 2023/1
N2 - Androgen assessment is a key element for diagnosing polycystic ovary syndrome (PCOS), and defining a “normal” level of circulating androgens is critical for epidemiological studies. We determined the upper normal limits (UNLs) for androgens in a population-based group of premenopausal “healthy control” women, overall and by ethnicity (Caucasian and Asian), in the cross-sectional Eastern Siberia PCOS Epidemiology and Phenotype (ESPEP) Study (ClinicalTrials.gov ID: NCT05194384) conducted in 2016–2019. Overall, we identified a “healthy control” group consisting of 143 healthy premenopausal women without menstrual dysfunction, hirsutism, polycystic ovaries, or medical disorders. We analyzed serum total testosterone (TT) by using liquid chromatography with tandem mass spectrometry (LC-MS/MS), and DHEAS, sex-hormone-binding globulin (SHBG), TSH, prolactin, and 17-hydroxyprogesterone (17OHP) were assessed with an enzyme-linked immunosorbent assay (ELISA). The UNLs for the entire population for the TT, free androgen index (FAI), and DHEAS were determined as the 98th percentiles in healthy controls as follows: 67.3 (95% confidence interval (CI): 48.1, 76.5) ng/dl, 5.4 (3.5, 14.0), and 355 (289, 371) μg/dl, respectively. The study results demonstrated that the UNLs for TT and FAI varied by ethnicity, whereas the DHEAS UNLs were comparable in the ethnicities studied.
AB - Androgen assessment is a key element for diagnosing polycystic ovary syndrome (PCOS), and defining a “normal” level of circulating androgens is critical for epidemiological studies. We determined the upper normal limits (UNLs) for androgens in a population-based group of premenopausal “healthy control” women, overall and by ethnicity (Caucasian and Asian), in the cross-sectional Eastern Siberia PCOS Epidemiology and Phenotype (ESPEP) Study (ClinicalTrials.gov ID: NCT05194384) conducted in 2016–2019. Overall, we identified a “healthy control” group consisting of 143 healthy premenopausal women without menstrual dysfunction, hirsutism, polycystic ovaries, or medical disorders. We analyzed serum total testosterone (TT) by using liquid chromatography with tandem mass spectrometry (LC-MS/MS), and DHEAS, sex-hormone-binding globulin (SHBG), TSH, prolactin, and 17-hydroxyprogesterone (17OHP) were assessed with an enzyme-linked immunosorbent assay (ELISA). The UNLs for the entire population for the TT, free androgen index (FAI), and DHEAS were determined as the 98th percentiles in healthy controls as follows: 67.3 (95% confidence interval (CI): 48.1, 76.5) ng/dl, 5.4 (3.5, 14.0), and 355 (289, 371) μg/dl, respectively. The study results demonstrated that the UNLs for TT and FAI varied by ethnicity, whereas the DHEAS UNLs were comparable in the ethnicities studied.
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U2 - 10.3390/diagnostics13010033
DO - 10.3390/diagnostics13010033
M3 - Article
C2 - 36611327
AN - SCOPUS:85145825543
SN - 2075-4418
VL - 13
JO - Diagnostics
JF - Diagnostics
IS - 1
M1 - 33
ER -