Estimation of protein adsorption on dialysis membrane by means of TOF-SIMS imaging

S. Aoyagi, M. Hayama, U. Hasegawa, K. Sakai, M. Tozu, T. Hoshi, M. Kudo

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28 Scopus citations


Protein adsorption on dialysis membrane was studied by time-of-flight secondary ion mass spectrometry (TOF-SIMS), which is expected to be capable of chemical imaging of insulated samples, such as hollow-fiber dialysis membranes. Three commercially available hollow-fiber dialysis membranes having different pore sizes and structures, were tested in the present study. Bovine serum albumin (BSA) solutions were used to adsorb the protein on the samples. TOF-SIMS images and spectra of native membranes and membranes treated with BSA were compared in order to identify secondary ions related to BSA and membranes. Mutual information, one of applications of information theory, was employed to select fragment ions related to BSA. TOF-SIMS images show distribution of adsorbed BSA on the dialysis membranes and indicate that BSA permeability and interaction between the membranes and BSA definitely depend on pore size, structure and material.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalJournal of Membrane Science
Issue number1-2
StatePublished - Jun 15 2004

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • General Materials Science
  • Physical and Theoretical Chemistry
  • Filtration and Separation


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