Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Camille Lebarbenchon; Mary Pantin-Jackwood; Whitney M. Kistler; M. Page Luttrell; Erica Spackman; David E. Stallknecht; Justin D. Brown

doi:10.1111/irv.12070

Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Camille Lebarbenchon
, Mary Pantin-Jackwood
, Whitney M. Kistler
, M. Page Luttrell
, Erica Spackman
, David E. Stallknecht
, Justin D. Brown

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.

Original language	English (US)
Pages (from-to)	1237-1240
Number of pages	4
Journal	Influenza and other Respiratory Viruses
Volume	7
Issue number	6
DOIs	https://doi.org/10.1111/irv.12070
State	Published - Nov 2013

All Science Journal Classification (ASJC) codes

Epidemiology
Pulmonary and Respiratory Medicine
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1111/irv.12070

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title = "Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl",

abstract = "The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.",

author = "Camille Lebarbenchon and Mary Pantin-Jackwood and Kistler, \{Whitney M.\} and \{Page Luttrell\}, M. and Erica Spackman and Stallknecht, \{David E.\} and Brown, \{Justin D.\}",

year = "2013",

month = nov,

doi = "10.1111/irv.12070",

language = "English (US)",

volume = "7",

pages = "1237--1240",

journal = "Influenza and other Respiratory Viruses",

issn = "1750-2640",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "6",

}

Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl. / Lebarbenchon, Camille; Pantin-Jackwood, Mary; Kistler, Whitney M. et al.
In: Influenza and other Respiratory Viruses, Vol. 7, No. 6, 11.2013, p. 1237-1240.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

AU - Lebarbenchon, Camille

AU - Pantin-Jackwood, Mary

AU - Kistler, Whitney M.

AU - Page Luttrell, M.

AU - Spackman, Erica

AU - Stallknecht, David E.

AU - Brown, Justin D.

PY - 2013/11

Y1 - 2013/11

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AB - The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.

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Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

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