Evaluation of multiple strains of Enterobacter sakazakii using fatty acid profiles

Maria Hoffmann; Christine E. Keys; Kwang Young Song; Eric Wayne Brown; Frederick S. Fry; Paul Whittaker

doi:10.1016/j.foodchem.2007.10.032

Evaluation of multiple strains of Enterobacter sakazakii using fatty acid profiles

Maria Hoffmann, Christine E. Keys, Kwang Young Song, Eric Wayne Brown, Frederick S. Fry, Paul Whittaker

Food Science

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Fatty acid profiles are useful for identifying Gram-negative Enterobacter sakazakii strains within the family Enterobacteriaceae. The majority of cases of E. sakazakii infection have involved sepsis, meningitis, or enteritis, especially in neonates and infants. Gas chromatography with flame ionization detection (GC-FID) was utilized for the analysis of cellular fatty acid methyl esters (FAMEs). Thirty E. sakazakii strains isolated from food and environmental sources were cultured for 24 h on brain heart infusion (BHI) agar on three different days at 37 °C. Whole cell FAMEs were obtained by saponification, methylation and extraction into hexane:methyl tert-butyl ether. The day to day variations for the 30 E. sakazakii strains for each fatty acid were determined. Gram-negative bacteria commonly contain combinations of straight-chain, unsaturated, hydroxyl, and cyclo fatty acids. Major fatty acids of E. sakazakii strains evaluated in this study were straight chain 12:0, 14:0, 16:0 and unsaturated 16:1, 18:1, and 17:0 ω cyclo 7-8. Analysis of FAMEs from E. sakazakii strains grown on BHI agar by this rapid GC-FID method is highly reproducible and provides a sensitive procedure for identification of this organism. The fatty acid profile assay could be used to rapidly screen infant formula samples for E. sakazakii and reduce the time required for the current assay by up to 5 days.

Original language	English (US)
Pages (from-to)	1623-1628
Number of pages	6
Journal	Food Chemistry
Volume	107
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2007.10.032
State	Published - Apr 15 2008

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2007.10.032

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title = "Evaluation of multiple strains of Enterobacter sakazakii using fatty acid profiles",

abstract = "Fatty acid profiles are useful for identifying Gram-negative Enterobacter sakazakii strains within the family Enterobacteriaceae. The majority of cases of E. sakazakii infection have involved sepsis, meningitis, or enteritis, especially in neonates and infants. Gas chromatography with flame ionization detection (GC-FID) was utilized for the analysis of cellular fatty acid methyl esters (FAMEs). Thirty E. sakazakii strains isolated from food and environmental sources were cultured for 24 h on brain heart infusion (BHI) agar on three different days at 37 °C. Whole cell FAMEs were obtained by saponification, methylation and extraction into hexane:methyl tert-butyl ether. The day to day variations for the 30 E. sakazakii strains for each fatty acid were determined. Gram-negative bacteria commonly contain combinations of straight-chain, unsaturated, hydroxyl, and cyclo fatty acids. Major fatty acids of E. sakazakii strains evaluated in this study were straight chain 12:0, 14:0, 16:0 and unsaturated 16:1, 18:1, and 17:0 ω cyclo 7-8. Analysis of FAMEs from E. sakazakii strains grown on BHI agar by this rapid GC-FID method is highly reproducible and provides a sensitive procedure for identification of this organism. The fatty acid profile assay could be used to rapidly screen infant formula samples for E. sakazakii and reduce the time required for the current assay by up to 5 days.",

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Evaluation of multiple strains of Enterobacter sakazakii using fatty acid profiles

Abstract

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