Evaluation of sampling and storage procedures on preserving the community structure of stool microbiota: A simple at-home toilet-paper collection method

Kait F. Al, Jordan E. Bisanz, Gregory B. Gloor, Gregor Reid, Jeremy P. Burton

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Background The increasing interest on the impact of the gut microbiota on health and disease has resulted in multiple human microbiome-related studies emerging. However, multiple sampling methods are being used, making cross-comparison of results difficult. To avoid additional clinic visits and increase patient recruitment to these studies, there is the potential to utilize at-home stool sampling. The aim of this pilot study was to compare simple self-sampling collection and storage methods. Methods To simulate storage conditions, stool samples from three volunteers were freshly collected, placed on toilet tissue, and stored at four temperatures (− 80, 7, 22 and 37 °C), either dry or in the presence of a stabilization agent (RNAlater®) for 3 or 7 days. Using 16S rRNA gene sequencing by Illumina, the effect of storage variations for each sample was compared to a reference community from fresh, unstored counterparts. Fastq files may be accessed in the NCBI Sequence Read Archive: Bioproject ID PRJNA418287. Results Microbial diversity and composition were not significantly altered by any storage method. Samples were always separable based on participant, regardless of storage method suggesting there was no need for sample preservation by a stabilization agent. Discussion In summary, if immediate sample processing is not feasible, short term storage of unpreserved stool samples on toilet paper offers a reliable way to assess the microbiota composition by 16S rRNA gene sequencing.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalJournal of Microbiological Methods
Volume144
DOIs
StatePublished - Jan 2018

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Evaluation of sampling and storage procedures on preserving the community structure of stool microbiota: A simple at-home toilet-paper collection method'. Together they form a unique fingerprint.

Cite this