Evaluation of the catalytic mechanism of AICAR transformylase by pH-dependent kinetics, mutagenesis, and quantum chemical calculations

Keyphrases

• Catalytic Mechanism 100%
• PH Dependence 100%
• Quantum Chemical Calculations 100%
• Mutagenesis 100%
• Carboxamide 100%
• AICAR Transformylase 100%
• Imidazole 88%
• AICAR 55%
• Amino Group 33%
• Amino 33%
• Formamide 33%
• Kcat 22%
• Ribonucleotides 22%
• General Acid 22%
• Acid Catalyst 22%
• 5-Aminoimidazoles 22%
• Positioning Accuracy 11%
• Site-directed mutagenesis 11%
• Km Value 11%
• Active Sites 11%
• Binding Affinity 11%
• Activation Energy 11%
• Transition State 11%
• Nucleophilic Attack 11%
• Proton Transfer 11%
• Burst Size 11%
• Protonation 11%
• Mutagenesis Studies 11%
• Stable Intermediate 11%
• Energy Barrier 11%
• Amides 11%
• Alternative Pathway 11%
• Hydrogen Bonding Interaction 11%
• Ammonium 11%
• PH Range 11%
• X-ray Structure 11%
• Base-free 11%
• B3LYP/6-31G 11%
• Mutant Enzyme 11%
• PH Profile 11%
• Intramolecular Proton Transfer 11%
• NMR Titration 11%

Chemistry

• Quantum Chemical Calculations 100%
• Carboxamide 100%
• Imidazole 100%
• Amino Group 37%
• Formamide 37%
• Proton Transfer 25%
• Kcat 25%
• Acid Catalyst 25%
• 5-Aminoimidazole-4-Carboxamide 25%
• Ribonucleotide 25%
• Chemistry 12%
• Base 12%
• Kd 12%
• NMR Spectroscopy 12%
• pKa 12%
• Reaction Activation Energy 12%
• Transition State 12%
• Energy Barrier 12%
• Hydrogen Bonding 12%
• DFT-B3LYP Calculation 12%
• Km 12%
• Site-Directed Mutagenesis 12%
• Cofactor 12%
• protonation 12%
• Arginine 12%