Evaluation of visible diffuse reflectance spectroscopy in liver tissue: Validation of tissue saturations using extracorporeal circulation

Stylianos Voulgarelis, Faraneh Fathi, Astrid G. Stucke, Kevin D. Daley, Joohyun Kim, Michael A. Zimmerman, Johnny C. Hong, Nicholas Starkey, Kenneth P. Allen, Bing Yu

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Significance: Real-time information about oxygen delivery to the hepatic graft is important to direct care and diagnose vascular compromise in the immediate post-transplant period. Aim: The current study was designed to determine the utility of visible diffuse reflectance spectroscopy (vis-DRS) for measuring liver tissue saturation in vivo. Approach: A custom-built vis-DRS probe was calibrated using phantoms with hemoglobin (Hb) and polystyrene microspheres. Ex vivo (extracorporeal circulation) and in vivo protocols were used in a swine model (n = 15) with validation via blood gas analysis. Results: In vivo absorption and scattering measured by vis-DRS with and without biliverdin correction correlated closely between analyses. Lin's concordance correlation coefficients are 0.991 for μa and 0.959 for μs ′ . Hb measured by blood test and vis-DRS with (R2 = 0.81) and without (R2 = 0.85) biliverdin correction were compared. Vis-DRS data obtained from the ex vivo protocol plotted against the PO2 derived from blood gas analysis showed a good fit for a Hill coefficient of 1.67 and P50 = 34 mmHg (R2 = 0.81). A conversion formula was developed to account for the systematic deviation, which resulted in a goodness-of-fit R2 = 0.76 with the expected oxygen dissociation curve. Conclusions: We show that vis-DRS allows for real-time measurement of liver tissue saturation, an indicator for liver perfusion and oxygen delivery.

Original languageEnglish (US)
Article number055002
JournalJournal of Biomedical Optics
Volume26
Issue number5
DOIs
StatePublished - May 1 2021

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials

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