Evidence for a direct and indirect action of leucine enkephalin at the feline ileocecal sphincter

Gerald Bertiger; Ann Ouyang; James C. Reynolds; Sidney Cohen

doi:10.1016/0024-3205(88)90035-5

Evidence for a direct and indirect action of leucine enkephalin at the feline ileocecal sphincter

Gerald Bertiger
, Ann Ouyang
, James C. Reynolds
, Sidney Cohen

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

An in vitro whole organ bath preparation was used to examine the effects of leucine enkephalin on the cat ileocecal sphincter (ICS) intraluminal pressure and myoelectric activity. The bath allowed separation of the bathing media surrounding the ICS and the ileum. Leucine enkephalin (2 × 10^-7M) when added to the ileal bathing medium caused a delayed increase in ICS spike activity and pressure which was blocked by tetrodotoxin (10^-5M). In contrast, leucine enkephalin (2 × 10^-7M) added directly to the ICS bathing medium caused an immediate spike-associated contractile response which was tetrodotoxin-resistant. Thus both an indirect and direct opiate action at the ICS was demonstrated.

Original language	English (US)
Pages (from-to)	1697-1703
Number of pages	7
Journal	Life Sciences
Volume	42
Issue number	18
DOIs	https://doi.org/10.1016/0024-3205(88)90035-5
State	Published - 1988

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
General Pharmacology, Toxicology and Pharmaceutics

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10.1016/0024-3205(88)90035-5

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title = "Evidence for a direct and indirect action of leucine enkephalin at the feline ileocecal sphincter",

abstract = "An in vitro whole organ bath preparation was used to examine the effects of leucine enkephalin on the cat ileocecal sphincter (ICS) intraluminal pressure and myoelectric activity. The bath allowed separation of the bathing media surrounding the ICS and the ileum. Leucine enkephalin (2 × 10-7M) when added to the ileal bathing medium caused a delayed increase in ICS spike activity and pressure which was blocked by tetrodotoxin (10-5M). In contrast, leucine enkephalin (2 × 10-7M) added directly to the ICS bathing medium caused an immediate spike-associated contractile response which was tetrodotoxin-resistant. Thus both an indirect and direct opiate action at the ICS was demonstrated.",

author = "Gerald Bertiger and Ann Ouyang and Reynolds, \{James C.\} and Sidney Cohen",

note = "Funding Information: This work was supported by NIH grants AM 19379-10 and AM 34148-03.",

year = "1988",

doi = "10.1016/0024-3205(88)90035-5",

language = "English (US)",

volume = "42",

pages = "1697--1703",

journal = "Life Sciences",

issn = "0024-3205",

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TY - JOUR

T1 - Evidence for a direct and indirect action of leucine enkephalin at the feline ileocecal sphincter

AU - Bertiger, Gerald

AU - Ouyang, Ann

AU - Reynolds, James C.

AU - Cohen, Sidney

N1 - Funding Information: This work was supported by NIH grants AM 19379-10 and AM 34148-03.

PY - 1988

Y1 - 1988

N2 - An in vitro whole organ bath preparation was used to examine the effects of leucine enkephalin on the cat ileocecal sphincter (ICS) intraluminal pressure and myoelectric activity. The bath allowed separation of the bathing media surrounding the ICS and the ileum. Leucine enkephalin (2 × 10-7M) when added to the ileal bathing medium caused a delayed increase in ICS spike activity and pressure which was blocked by tetrodotoxin (10-5M). In contrast, leucine enkephalin (2 × 10-7M) added directly to the ICS bathing medium caused an immediate spike-associated contractile response which was tetrodotoxin-resistant. Thus both an indirect and direct opiate action at the ICS was demonstrated.

AB - An in vitro whole organ bath preparation was used to examine the effects of leucine enkephalin on the cat ileocecal sphincter (ICS) intraluminal pressure and myoelectric activity. The bath allowed separation of the bathing media surrounding the ICS and the ileum. Leucine enkephalin (2 × 10-7M) when added to the ileal bathing medium caused a delayed increase in ICS spike activity and pressure which was blocked by tetrodotoxin (10-5M). In contrast, leucine enkephalin (2 × 10-7M) added directly to the ICS bathing medium caused an immediate spike-associated contractile response which was tetrodotoxin-resistant. Thus both an indirect and direct opiate action at the ICS was demonstrated.

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UR - https://www.scopus.com/pages/publications/0023935366#tab=citedBy

U2 - 10.1016/0024-3205(88)90035-5

DO - 10.1016/0024-3205(88)90035-5

M3 - Article

C2 - 2896285

AN - SCOPUS:0023935366

SN - 0024-3205

VL - 42

SP - 1697

EP - 1703

JO - Life Sciences

JF - Life Sciences

IS - 18

ER -

Evidence for a direct and indirect action of leucine enkephalin at the feline ileocecal sphincter

Abstract

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