Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes

Juan Du, Venu Gopal Vandavasi, Kelly R. Molloy, Hui Yang, Lynnicia N. Massenburg, Abhishek Singh, Albert L. Kwansa, Yaroslava G. Yingling, Hugh O'Neill, Brian T. Chait, Manish Kumar, B. Tracy Nixon

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Higher plants synthesize cellulose using membrane-bound, six-lobed cellulose synthase complexes, each lobe containing trimeric cellulose synthases (CESAs). Although molecular biology reports support heteromeric trimers composed of different isoforms, a homomeric trimer was reported for in vitro studies of the catalytic domain of CESA1 of Arabidopsis (AtCESA1CatD) and confirmed in cryoEM structures of full-length CESA8 and CESA7 of poplar and cotton, respectively. In both structures, a small portion of the plant-conserved region (P-CR) forms the only contacts between catalytic domains of the monomers. We report inter-subunit lysine-crosslinks that localize to the small P-CR, negative-stain EM structure, and modeling data for homotrimers of AtCESA1CatD. Molecular dynamics simulations for AtCESA1CatD trimers based on the CESA8 cryoEM structure were stable and dependent upon a small set of residue contacts. The results suggest that homomeric CESA trimers may be important for the synthesis of primary and secondary cell walls and identify key residues for future mutagenic studies.

Original languageEnglish (US)
Pages (from-to)3663-3677
Number of pages15
Issue number9
StatePublished - Sep 12 2022

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry


Dive into the research topics of 'Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes'. Together they form a unique fingerprint.

Cite this