Evidence for Progesterone Receptors in Human Osteoblast-like Cells

Lisa L. Wei; Mark W. Leach; Robyn S. Miner; Laurence M. Demers

doi:10.1006/bbrc.1993.2077

Evidence for Progesterone Receptors in Human Osteoblast-like Cells

Lisa L. Wei, Mark W. Leach, Robyn S. Miner, Laurence M. Demers

Department of Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We report on the presence of progesterone receptor and its mRNA in a human osteoblast-like cell line (HOS TE85) using recombinant DNA methods, saturation analysis, specific immunoprecipitation with an anti-progesterone receptor antibody, and Northern blot analysis. These osteoblast-like cells were also found to be responsive to progesterone in a dose-dependent fashion through an augmentation in cellular alkaline phosphatase activity. These results support the presence of progesterone receptors in bone cells and suggest a direct effort of progesterone and/or antiprogestins on osteoblast cell function.

Original language	English (US)
Pages (from-to)	525-532
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	195
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1993.2077
State	Published - Sep 15 1993

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1993.2077

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AB - We report on the presence of progesterone receptor and its mRNA in a human osteoblast-like cell line (HOS TE85) using recombinant DNA methods, saturation analysis, specific immunoprecipitation with an anti-progesterone receptor antibody, and Northern blot analysis. These osteoblast-like cells were also found to be responsive to progesterone in a dose-dependent fashion through an augmentation in cellular alkaline phosphatase activity. These results support the presence of progesterone receptors in bone cells and suggest a direct effort of progesterone and/or antiprogestins on osteoblast cell function.

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Evidence for Progesterone Receptors in Human Osteoblast-like Cells

Abstract

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