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Evidence for Progesterone Receptors in Human Osteoblast-like Cells

  • Lisa L. Wei
  • , Mark W. Leach
  • , Robyn S. Miner
  • , Laurence M. Demers

Research output: Contribution to journalArticlepeer-review

Abstract

We report on the presence of progesterone receptor and its mRNA in a human osteoblast-like cell line (HOS TE85) using recombinant DNA methods, saturation analysis, specific immunoprecipitation with an anti-progesterone receptor antibody, and Northern blot analysis. These osteoblast-like cells were also found to be responsive to progesterone in a dose-dependent fashion through an augmentation in cellular alkaline phosphatase activity. These results support the presence of progesterone receptors in bone cells and suggest a direct effort of progesterone and/or antiprogestins on osteoblast cell function.

Original languageEnglish (US)
Pages (from-to)525-532
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number2
DOIs
StatePublished - Sep 15 1993

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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