Evidence for transmission of Plasmodium vivax among a Duffy antigen negative population in Western Kenya

Jeffrey R. Ryan; José A. Stoute; Joseph Amon; Raymond F. Dunton; Ramadhan Mtalib; Joseph Koros; Boaz Owour; Shirley Luckhart; Robert A. Wirtz; John W. Barnwell; Ronald Rosenberg

doi:10.4269/ajtmh.2006.75.575

Evidence for transmission of Plasmodium vivax among a Duffy antigen negative population in Western Kenya

Jeffrey R. Ryan, José A. Stoute, Joseph Amon, Raymond F. Dunton, Ramadhan Mtalib, Joseph Koros, Boaz Owour, Shirley Luckhart, Robert A. Wirtz, John W. Barnwell, Ronald Rosenberg

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179 Scopus citations

Abstract

We present evidence that a parasite with characteristics of Plasmodium vivax is being transmitted among Duffy blood group-negative inhabitants of Kenya. Thirty-two of 4,901 Anopheles gambiae and An. funestus (0.65%) collected in Nyanza Province were ELISA positive for the P. vivax circumsporozoite protein VK 247. AH positives were found late in the rainy season, when An. funestus predominated, and disproportionately many were found at a single village. A P. vivax specific sequence of the SSU rRNA gene was amplified from three of six ELISA-positive mosquitoes. Erythrocytes from 31 children, including 9 microscopically diagnosed as infected with P. vivax, were negative by flow cytometry for the Fy3 or Fy6 epitopes, which indicate Duffy blood group expression. A DNA fragment specific for the C terminus of the gene for P. vivax merozoite surface protein 1 (MSP-1) was amplified from the blood of four of these children and subsequently sequenced from two.

Original language	English (US)
Pages (from-to)	575-581
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	75
Issue number	4
DOIs	https://doi.org/10.4269/ajtmh.2006.75.575
State	Published - 2006

All Science Journal Classification (ASJC) codes

Parasitology
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4269/ajtmh.2006.75.575

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Ryan, J. R., Stoute, J. A., Amon, J., Dunton, R. F., Mtalib, R., Koros, J., Owour, B., Luckhart, S., Wirtz, R. A., Barnwell, J. W., & Rosenberg, R. (2006). Evidence for transmission of Plasmodium vivax among a Duffy antigen negative population in Western Kenya. American Journal of Tropical Medicine and Hygiene, 75(4), 575-581. https://doi.org/10.4269/ajtmh.2006.75.575

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abstract = "We present evidence that a parasite with characteristics of Plasmodium vivax is being transmitted among Duffy blood group-negative inhabitants of Kenya. Thirty-two of 4,901 Anopheles gambiae and An. funestus (0.65%) collected in Nyanza Province were ELISA positive for the P. vivax circumsporozoite protein VK 247. AH positives were found late in the rainy season, when An. funestus predominated, and disproportionately many were found at a single village. A P. vivax specific sequence of the SSU rRNA gene was amplified from three of six ELISA-positive mosquitoes. Erythrocytes from 31 children, including 9 microscopically diagnosed as infected with P. vivax, were negative by flow cytometry for the Fy3 or Fy6 epitopes, which indicate Duffy blood group expression. A DNA fragment specific for the C terminus of the gene for P. vivax merozoite surface protein 1 (MSP-1) was amplified from the blood of four of these children and subsequently sequenced from two.",

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AU - Ryan, Jeffrey R.

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AU - Dunton, Raymond F.

AU - Mtalib, Ramadhan

AU - Koros, Joseph

AU - Owour, Boaz

AU - Luckhart, Shirley

AU - Wirtz, Robert A.

AU - Barnwell, John W.

AU - Rosenberg, Ronald

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N2 - We present evidence that a parasite with characteristics of Plasmodium vivax is being transmitted among Duffy blood group-negative inhabitants of Kenya. Thirty-two of 4,901 Anopheles gambiae and An. funestus (0.65%) collected in Nyanza Province were ELISA positive for the P. vivax circumsporozoite protein VK 247. AH positives were found late in the rainy season, when An. funestus predominated, and disproportionately many were found at a single village. A P. vivax specific sequence of the SSU rRNA gene was amplified from three of six ELISA-positive mosquitoes. Erythrocytes from 31 children, including 9 microscopically diagnosed as infected with P. vivax, were negative by flow cytometry for the Fy3 or Fy6 epitopes, which indicate Duffy blood group expression. A DNA fragment specific for the C terminus of the gene for P. vivax merozoite surface protein 1 (MSP-1) was amplified from the blood of four of these children and subsequently sequenced from two.

AB - We present evidence that a parasite with characteristics of Plasmodium vivax is being transmitted among Duffy blood group-negative inhabitants of Kenya. Thirty-two of 4,901 Anopheles gambiae and An. funestus (0.65%) collected in Nyanza Province were ELISA positive for the P. vivax circumsporozoite protein VK 247. AH positives were found late in the rainy season, when An. funestus predominated, and disproportionately many were found at a single village. A P. vivax specific sequence of the SSU rRNA gene was amplified from three of six ELISA-positive mosquitoes. Erythrocytes from 31 children, including 9 microscopically diagnosed as infected with P. vivax, were negative by flow cytometry for the Fy3 or Fy6 epitopes, which indicate Duffy blood group expression. A DNA fragment specific for the C terminus of the gene for P. vivax merozoite surface protein 1 (MSP-1) was amplified from the blood of four of these children and subsequently sequenced from two.

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Evidence for transmission of Plasmodium vivax among a Duffy antigen negative population in Western Kenya

Abstract

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