TY - JOUR
T1 - Experimental and kinetic methods for studying vitamin A dynamics in vivo
AU - Green, Michael H.
AU - Green, Joanne Balmer
N1 - Funding Information:
Work in the laboratory ofM. H. Green was supported by U.S. Department of Agriculture Competitive Research Grants 81-CRCR-1-0702 and 88-37200-3537. We acknowledge Barbara A. Underwood who introduced us to the exciting field of vitamin A dynamics through a collaborative research project.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - Many types of kinetic studies have been done to investigate the metabolism of retinoids. This chapter reviews the methods used to study vitamin A dynamics in the rat. The approach has been to administer radioactive vitamin A in a physiological form, so that tracer data can be extrapolated to the tracees of interest (retinol and retinyl esters). The chapter applies several kinetic methods to analyze the in vivo data, with the goal of developing a quantitative description of whole-body vitamin A dynamics at different levels of vitamin A status. Such techniques are applicable to studies of vitamin A metabolism in different physiological or nutritional states using rats or other species, as well as to experiments on the effects of alcohol and xenobiotics on vitamin A metabolism and to those on disposition of pharmacologically useful retinoids.
AB - Many types of kinetic studies have been done to investigate the metabolism of retinoids. This chapter reviews the methods used to study vitamin A dynamics in the rat. The approach has been to administer radioactive vitamin A in a physiological form, so that tracer data can be extrapolated to the tracees of interest (retinol and retinyl esters). The chapter applies several kinetic methods to analyze the in vivo data, with the goal of developing a quantitative description of whole-body vitamin A dynamics at different levels of vitamin A status. Such techniques are applicable to studies of vitamin A metabolism in different physiological or nutritional states using rats or other species, as well as to experiments on the effects of alcohol and xenobiotics on vitamin A metabolism and to those on disposition of pharmacologically useful retinoids.
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U2 - 10.1016/0076-6879(90)90035-Y
DO - 10.1016/0076-6879(90)90035-Y
M3 - Article
C2 - 2087182
AN - SCOPUS:0025606708
SN - 0076-6879
VL - 190
SP - 304
EP - 317
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -