Abstract
Given the explosion in genomic information, the historical “one gene at a time” approach to gene expression analysis is no longer adequate. Instead, large-scale multiplex methods for analyzing gene expression patterns are needed. Several technologies have been developed to serve this function, including differential display, serial analysis of gene expression (SAGE), total gene expression analysis (TOGA), subtraction cloning, and DNA hybridization arrays (microarrays). 1 This last approach, which is rapidly becoming the dominant technology in the gene expression field, is the subject of this chapter. However, this powerful new technology also comes with a unique set of considerations when it comes to designing and executing experiments. In this chapter, experimental design will be considered from both strategic and tactical standpoints.
Original language | English (US) |
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Title of host publication | DNA Arrays |
Subtitle of host publication | Technologies and Experimental Strategies |
Publisher | CRC Press |
Pages | 11-24 |
Number of pages | 14 |
ISBN (Electronic) | 9781420038859 |
ISBN (Print) | 0849322855, 9780849322853 |
State | Published - Jan 1 2001 |
All Science Journal Classification (ASJC) codes
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology