Experimental design for hybridization array analysis of gene expression

Willard M. Freeman, Kent Vrana

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Given the explosion in genomic information, the historical “one gene at a time” approach to gene expression analysis is no longer adequate. Instead, large-scale multiplex methods for analyzing gene expression patterns are needed. Several technologies have been developed to serve this function, including differential display, serial analysis of gene expression (SAGE), total gene expression analysis (TOGA), subtraction cloning, and DNA hybridization arrays (microarrays). 1 This last approach, which is rapidly becoming the dominant technology in the gene expression field, is the subject of this chapter. However, this powerful new technology also comes with a unique set of considerations when it comes to designing and executing experiments. In this chapter, experimental design will be considered from both strategic and tactical standpoints.

Original languageEnglish (US)
Title of host publicationDNA Arrays
Subtitle of host publicationTechnologies and Experimental Strategies
PublisherCRC Press
Number of pages14
ISBN (Electronic)9781420038859
ISBN (Print)0849322855, 9780849322853
StatePublished - Jan 1 2001

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology


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