Experimental infection of eastern gray squirrels (Sciurus carolinensis) with West Nile virus

Andrés Gómez, Laura D. Kramer, Alan P. Dupuis, A. Marm Kilpatrick, Lauren J. Davis, Matthew J. Jones, Peter Daszak, A. Alonso Aguirre

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Eastern gray squirrels (Sciurus carolinensis) have shown high West Nile virus (WNV) seroprevalence, and WNV infection has been suggested as a cause of morbidity and mortality in this species. We experimentally infected nine eastern gray squirrels with WNV to determine the clinical effects of infection and to assess their potential role as amplifying hosts. We observed no morbidity or mortality attributable to WNV infection, but lesions were apparent in several organs. We detected mean viremias of 105.1 and 104.8 plaque-forming units (PFU)/mL on days 3 and 4 post-infection (DPI) and estimated that ∼2.1% of Culex pipiens feeding on squirrels during 1-5 DPI would become infectious. Thus, S. carolinensis are unlikely to be important amplifying hosts and may instead dampen the intensity of transmission in most host communities. The low viremias and lack of mortality observed in S. carolinensis suggest that they may be useful as sentinels of spillover from the enzootic amplification cycle.

Original languageEnglish (US)
Pages (from-to)447-451
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number3
StatePublished - Sep 2008

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology
  • Parasitology


Dive into the research topics of 'Experimental infection of eastern gray squirrels (Sciurus carolinensis) with West Nile virus'. Together they form a unique fingerprint.

Cite this