Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa

Mary Anne Nelson, Seogchan Kang, Edward L. Braun, Matthew E. Crawford, Patricia L. Dolan, Pascale M. Leonard, Jason Mitchell, Anne Marie Armijo, Laura Bean, Eldon Blueyes, Thomas Cushing, Allison Errett, Mark Fleharty, Marianita Gorman, Kimberly Judson, Robert Miller, Jennifer Ortega, Iglika Pavlova, John Perea, Shanda TodiscoRobert Trujillo, Joseph Valentine, Audrey Wells, Margaret Werner-Washburne, Sheldwin Yazzie, Donald O. Natvig

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125 Scopus citations


In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1865 partial complementary DNA (cDNA) sequences for 1409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34% of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56% of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3% were involved in metabolism, 32.9% in protein synthesis and 8.4% in RNA synthesis. Fewer were involved in defense (6%), cell signalling (3.4%), cell structure (3.4%) and cell division (2.6%).

Original languageEnglish (US)
Pages (from-to)348-363
Number of pages16
JournalFungal Genetics and Biology
Issue number3
StatePublished - Jun 1997

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics


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