@article{2cdc5b27669647a080f06199913018ca,
title = "Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa",
abstract = "In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1865 partial complementary DNA (cDNA) sequences for 1409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34% of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56% of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3% were involved in metabolism, 32.9% in protein synthesis and 8.4% in RNA synthesis. Fewer were involved in defense (6%), cell signalling (3.4%), cell structure (3.4%) and cell division (2.6%).",
author = "Nelson, {Mary Anne} and Seogchan Kang and Braun, {Edward L.} and Crawford, {Matthew E.} and Dolan, {Patricia L.} and Leonard, {Pascale M.} and Jason Mitchell and Armijo, {Anne Marie} and Laura Bean and Eldon Blueyes and Thomas Cushing and Allison Errett and Mark Fleharty and Marianita Gorman and Kimberly Judson and Robert Miller and Jennifer Ortega and Iglika Pavlova and John Perea and Shanda Todisco and Robert Trujillo and Joseph Valentine and Audrey Wells and Margaret Werner-Washburne and Sheldwin Yazzie and Natvig, {Donald O.}",
note = "Funding Information: We thank Matthew Sachs for providing the mRNA used in construction of the germinated conidial cDNA library and for helpful comments. This research was supported by the National Science Foundation RIMI (Research Improvements in Minority Institutions) Program Grant HRD-9550649 to D.O.N., M.A.N., M.W.W., and R.M., the U.S. Public Health Service Grant GM47374 to M.A.N., and matching funds from the University of New Mexico. A.M.A., J.P., and R.T. received support from the National Institutes of Health Minority Biomedical Research Support program (GM52576), and L.B. and J.V. acknowledge support from the Minorities in Engineering, Mathematics and the Sciences Program. The Howard Hughes Undergraduate Research Program at UNM supported J.M., T.C., A.E., K.J., I.P., S.T., and A.W. E.B., M.G., J.O., and S.Y. were supported by the RIMI Award. Initial support of J.O. and S.Y. was from the UNM Research Opportunities Program.",
year = "1997",
month = jun,
doi = "10.1006/fgbi.1997.0986",
language = "English (US)",
volume = "21",
pages = "348--363",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",
number = "3",
}