Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

Stanley Person; Susan C. Warner; David J. Bzik; Chitrita Debroy; Barbara A. Fox

doi:10.1016/0378-1119(85)90006-X

Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

Stanley Person, Susan C. Warner, David J. Bzik, Chitrita Debroy, Barbara A. Fox

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

Original language	English (US)
Pages (from-to)	279-287
Number of pages	9
Journal	Gene
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/0378-1119(85)90006-X
State	Published - 1985

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/0378-1119(85)90006-X

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title = "Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2",

abstract = "A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].",

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year = "1985",

doi = "10.1016/0378-1119(85)90006-X",

language = "English (US)",

volume = "35",

pages = "279--287",

journal = "Gene",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

AU - Person, Stanley

AU - Warner, Susan C.

AU - Bzik, David J.

AU - Debroy, Chitrita

AU - Fox, Barbara A.

PY - 1985

Y1 - 1985

N2 - A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

AB - A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

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AN - SCOPUS:0022263096

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EP - 287

JO - Gene

JF - Gene

IS - 3

ER -

Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

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