TY - JOUR
T1 - Expression of 1,25-dihydroxyvitamin D3 receptor in the immune system
AU - Veldman, Christian M.
AU - Cantorna, Margherita T.
AU - DeLuca, Hector F.
N1 - Funding Information:
1This work was supported in part by a Program Project Grant DK14881 from the National Institutes of Health and a fund from the Wisconsin Alumni Research Foundation. 2Present address: Nutrition Department, Penn State University, University Park, PA 16802. 3To whom correspondence should be addressed.
PY - 2000/2/15
Y1 - 2000/2/15
N2 - In addition to its role in calcium and skeletal homeostasis, there is increasing evidence that the hormonal form of vitamin D, 1,25- dihydroxyvitamin D3, appears to serve as a modulator of the immune system. We have determined the level of the 1,25-dihydroxyvitamin D3 receptor (VDR) in resting and activated lymphocytes by immuno- and ligand-binding assays. As expected from previous work, the total T lymphocyte population contains VDR whose levels are increased when activated and treated with 1,25- dihydroxyvitamin D3. Surprisingly, the highest concentrations of VDR are found in CD8 lymphocytes, although significant amounts are also present in CD4 lymphocytes. Furthermore, B lymphocytes do not contain detectable amounts of VDR. Cells of the monocyte/macrophage lineage possess small amounts of VDR that are not affected by activation but are increased by treatment with 1,25- dihydroxyvitamin D3. These results suggest that CD8 lymphocytes may be a major site of 1,25-dihydroxyvitamin D3 action, while B lymphocytes are likely not directly regulated by 1,25-dihydroxyvitamin D3. (C) 2000 Academic Press.
AB - In addition to its role in calcium and skeletal homeostasis, there is increasing evidence that the hormonal form of vitamin D, 1,25- dihydroxyvitamin D3, appears to serve as a modulator of the immune system. We have determined the level of the 1,25-dihydroxyvitamin D3 receptor (VDR) in resting and activated lymphocytes by immuno- and ligand-binding assays. As expected from previous work, the total T lymphocyte population contains VDR whose levels are increased when activated and treated with 1,25- dihydroxyvitamin D3. Surprisingly, the highest concentrations of VDR are found in CD8 lymphocytes, although significant amounts are also present in CD4 lymphocytes. Furthermore, B lymphocytes do not contain detectable amounts of VDR. Cells of the monocyte/macrophage lineage possess small amounts of VDR that are not affected by activation but are increased by treatment with 1,25- dihydroxyvitamin D3. These results suggest that CD8 lymphocytes may be a major site of 1,25-dihydroxyvitamin D3 action, while B lymphocytes are likely not directly regulated by 1,25-dihydroxyvitamin D3. (C) 2000 Academic Press.
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U2 - 10.1006/abbi.1999.1605
DO - 10.1006/abbi.1999.1605
M3 - Article
C2 - 10666315
AN - SCOPUS:0034652314
SN - 0003-9861
VL - 374
SP - 334
EP - 338
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -