TY - JOUR
T1 - Expression of Bacillus cereus hemolysin II in Bacillus subtilis renders the bacteria pathogenic for the crustacean daphnia magna
AU - Sineva, Elena V.
AU - Andreeva-Kovalevskaya, Zhanna I.
AU - Shadrin, Andrey M.
AU - Gerasimov, Yury L.
AU - Ternovsky, Vadim I.
AU - Teplova, Vera V.
AU - Yurkova, Tatyana V.
AU - Solonin, Alexander S.
PY - 2009/10
Y1 - 2009/10
N2 - Hemolysin II (HlyII) is a pore-forming toxin of the opportunistic pathogen Bacillus cereus. Despite our understanding of the mechanism of HlyII cytotoxicity in vitro, many of its characteristics, including potential target cells, conditions of its action and expression, are not known. Here we report that the expression of hlyII in Bacillus subtilis renders the bacteria hemolytic and is able to kill the crustacean Daphnia magna. The hemolytic activity of hlyII-encoded B. subtilis strains in culture media is positively correlated with virulence in D. magna. Fluorescence microscopy reveals postinfection changes in the mitochondrial potential of intestinal tissue, suggesting that the formation of ionic pores leads to cell death. In the presence of the transcriptional regulator HlyIIR, HlyII expression decreases 200-fold, and B. subtilis expressing both hlyII and hlyIIR remains hemolytic, but not pathogenic to the crustacean.
AB - Hemolysin II (HlyII) is a pore-forming toxin of the opportunistic pathogen Bacillus cereus. Despite our understanding of the mechanism of HlyII cytotoxicity in vitro, many of its characteristics, including potential target cells, conditions of its action and expression, are not known. Here we report that the expression of hlyII in Bacillus subtilis renders the bacteria hemolytic and is able to kill the crustacean Daphnia magna. The hemolytic activity of hlyII-encoded B. subtilis strains in culture media is positively correlated with virulence in D. magna. Fluorescence microscopy reveals postinfection changes in the mitochondrial potential of intestinal tissue, suggesting that the formation of ionic pores leads to cell death. In the presence of the transcriptional regulator HlyIIR, HlyII expression decreases 200-fold, and B. subtilis expressing both hlyII and hlyIIR remains hemolytic, but not pathogenic to the crustacean.
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U2 - 10.1111/j.1574-6968.2009.01742.x
DO - 10.1111/j.1574-6968.2009.01742.x
M3 - Article
C2 - 19686345
AN - SCOPUS:69949175896
SN - 0378-1097
VL - 299
SP - 110
EP - 119
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -