TY - JOUR
T1 - Expression of epidermal growth factor and platelet-derived growth factor receptors during cervical carcinogenesis
AU - Mayer, T. J.
AU - Frauenhoffer, E. E.
AU - Meyers, C.
N1 - Funding Information:
We thank Lyn Budgeon, Karen Wagner, and Danuta Huber for excellent technical assistance. We are grateful to Michelle A. Ozbun and Jennifer Bromberg for critical reading of the manuscript. This work was supported by NIH Training Grant CA60395 (T. J. M.) and by NIH Grant CA-64624 (C.
PY - 2000
Y1 - 2000
N2 - Altered expression of epidermal growth factor receptor (EGFR) is common in a variety of epithelial malignancies, including cervical cancer. However, the prognostic significance of EGFR expression is controversial for cervical cancer. Platelet-derived growth factor receptor (PDGFR) expression status is unknown in cervical cancer. Our results demonstrated that expression of EGFR and PDGFR was greatly enhanced in vivo and in organotypic cultures of low-grade cervical dysplastic tissues, but levels were decreased in high-grade lesions. To our knowledge, this is the first report identifying the expression of PDGFR in human epithelium. When low-grade dysplastic organotypic culture tissues were induced to differentiate more completely, EGFR expression, but not PDGFR expression, was relocalized to the basal layer as seen in normal tissues. Differentiation also induced phosphorylation of EGFR but not PDGFR. Our results suggest a role for EGFR and PDGFR during the early stages of cervical carcinogenesis, and demonstrate the facility of organotypic cultures to study the role of these growth factors in the development-of cervical cancer.
AB - Altered expression of epidermal growth factor receptor (EGFR) is common in a variety of epithelial malignancies, including cervical cancer. However, the prognostic significance of EGFR expression is controversial for cervical cancer. Platelet-derived growth factor receptor (PDGFR) expression status is unknown in cervical cancer. Our results demonstrated that expression of EGFR and PDGFR was greatly enhanced in vivo and in organotypic cultures of low-grade cervical dysplastic tissues, but levels were decreased in high-grade lesions. To our knowledge, this is the first report identifying the expression of PDGFR in human epithelium. When low-grade dysplastic organotypic culture tissues were induced to differentiate more completely, EGFR expression, but not PDGFR expression, was relocalized to the basal layer as seen in normal tissues. Differentiation also induced phosphorylation of EGFR but not PDGFR. Our results suggest a role for EGFR and PDGFR during the early stages of cervical carcinogenesis, and demonstrate the facility of organotypic cultures to study the role of these growth factors in the development-of cervical cancer.
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U2 - 10.1290/1071-2690(2000)036<0667:EOEGFA>2.0.CO;2
DO - 10.1290/1071-2690(2000)036<0667:EOEGFA>2.0.CO;2
M3 - Article
C2 - 11229599
AN - SCOPUS:0034468599
SN - 1071-2690
VL - 36
SP - 667
EP - 676
JO - In Vitro Cellular and Developmental Biology - Animal
JF - In Vitro Cellular and Developmental Biology - Animal
IS - 10
ER -