TY - JOUR
T1 - Expression of FOXP3 in CD4+ CD39+ T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid
AU - Li, Dong Mei
AU - Li, Xiang Pei
AU - Li, Xiao Mei
AU - Wang, Guo Sheng
AU - Ma, Yan
AU - Zhao, Shu Shan
AU - Zheng, Song Guo
PY - 2009/6/16
Y1 - 2009/6/16
N2 - Objective: To investigate the level of FOXP3 expressed in CD4+ CD39+ T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4+ CD25+ CD39+, CD4+ CD25+ FOXP3+ and CD4+ CD39+ FOXP3+ T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4+ CD25+ CD39+ T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4+ CD25+, CD4+ CD25high and CD4+ CD39+ T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4+ CD25high and CD4+ CD39+ T cells were higher than that expressed in CD4+ CD25+ T cells (P < 0.01), while it had no significant difference between CD4+ CD25highT cells and CD4+ CD39+ T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39+ Treg cells may play an important role in the pathogenesis of SLE.
AB - Objective: To investigate the level of FOXP3 expressed in CD4+ CD39+ T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4+ CD25+ CD39+, CD4+ CD25+ FOXP3+ and CD4+ CD39+ FOXP3+ T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4+ CD25+ CD39+ T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4+ CD25+, CD4+ CD25high and CD4+ CD39+ T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4+ CD25high and CD4+ CD39+ T cells were higher than that expressed in CD4+ CD25+ T cells (P < 0.01), while it had no significant difference between CD4+ CD25highT cells and CD4+ CD39+ T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39+ Treg cells may play an important role in the pathogenesis of SLE.
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U2 - 10.3760/cma.j.issn.0376-2491.2009.23.015
DO - 10.3760/cma.j.issn.0376-2491.2009.23.015
M3 - Article
C2 - 19957514
AN - SCOPUS:84871271882
SN - 0376-2491
VL - 89
SP - 1636
EP - 1638
JO - National Medical Journal of China
JF - National Medical Journal of China
IS - 23
ER -