TY - JOUR
T1 -
Expression of FOXP3 in CD4
+
CD39
+
T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid
AU - Li, Dong Mei
AU - Li, Xiang Pei
AU - Li, Xiao Mei
AU - Wang, Guo Sheng
AU - Ma, Yan
AU - Zhao, Shu Shan
AU - Zheng, Song Guo
PY - 2009/6/16
Y1 - 2009/6/16
N2 -
Objective: To investigate the level of FOXP3 expressed in CD4
+
CD39
+
T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4
+
CD25
+
CD39
+
, CD4
+
CD25
+
FOXP3
+
and CD4
+
CD39
+
FOXP3
+
T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4
+
CD25
+
CD39
+
T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4
+
CD25
+
, CD4
+
CD25
high
and CD4
+
CD39
+
T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4
+
CD25
high
and CD4
+
CD39
+
T cells were higher than that expressed in CD4
+
CD25
+
T cells (P < 0.01), while it had no significant difference between CD4
+
CD25
high
T cells and CD4
+
CD39
+
T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39
+
Treg cells may play an important role in the pathogenesis of SLE.
AB -
Objective: To investigate the level of FOXP3 expressed in CD4
+
CD39
+
T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4
+
CD25
+
CD39
+
, CD4
+
CD25
+
FOXP3
+
and CD4
+
CD39
+
FOXP3
+
T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4
+
CD25
+
CD39
+
T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4
+
CD25
+
, CD4
+
CD25
high
and CD4
+
CD39
+
T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4
+
CD25
high
and CD4
+
CD39
+
T cells were higher than that expressed in CD4
+
CD25
+
T cells (P < 0.01), while it had no significant difference between CD4
+
CD25
high
T cells and CD4
+
CD39
+
T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39
+
Treg cells may play an important role in the pathogenesis of SLE.
UR - http://www.scopus.com/inward/record.url?scp=84871271882&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871271882&partnerID=8YFLogxK
U2 - 10.3760/cma.j.issn.0376-2491.2009.23.015
DO - 10.3760/cma.j.issn.0376-2491.2009.23.015
M3 - Article
C2 - 19957514
AN - SCOPUS:84871271882
SN - 0376-2491
VL - 89
SP - 1636
EP - 1638
JO - National Medical Journal of China
JF - National Medical Journal of China
IS - 23
ER -