Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger Mouse

Masahito Kamanaka; Sean T. Kim; Yisong Y. Wan; Fayyaz S. Sutterwala; Maria Lara-Tejero; Jorge E. Galán; Ed Harhaj; Richard A. Flavell

doi:10.1016/j.immuni.2006.09.013

Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger Mouse

Masahito Kamanaka, Sean T. Kim, Yisong Y. Wan, Fayyaz S. Sutterwala, Maria Lara-Tejero, Jorge E. Galán, Ed Harhaj, Richard A. Flavell

Research output: Contribution to journal › Article › peer-review

338 Scopus citations

Abstract

To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.

Original language	English (US)
Pages (from-to)	941-952
Number of pages	12
Journal	Immunity
Volume	25
Issue number	6
DOIs	https://doi.org/10.1016/j.immuni.2006.09.013
State	Published - Dec 2006

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Infectious Diseases

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10.1016/j.immuni.2006.09.013

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title = "Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger Mouse",

abstract = "To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.",

author = "Masahito Kamanaka and Kim, {Sean T.} and Wan, {Yisong Y.} and Sutterwala, {Fayyaz S.} and Maria Lara-Tejero and Gal{\'a}n, {Jorge E.} and Ed Harhaj and Flavell, {Richard A.}",

note = "Funding Information: We thank F. Manzo for assistance with manuscript preparation, L. Evangelisti and C. Hughes for generating ES cells and chimeric mice, respectively, J. Stein for initial screening of tiger mice by Southern, and E. Eynon for managing the mouse program. We thank A. Iwasaki for help with isolation of Peyer's patch DC. This work was supported by NIH AI36529 (R.A.F.). Work in J.E.G.'s laboratory is supported by Public Health Service Grant AI055472. R.A.F. is an investigator of the Howard Hughes Medical Institute. ",

year = "2006",

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language = "English (US)",

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journal = "Immunity",

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T1 - Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger Mouse

AU - Kamanaka, Masahito

AU - Kim, Sean T.

AU - Wan, Yisong Y.

AU - Sutterwala, Fayyaz S.

AU - Lara-Tejero, Maria

AU - Galán, Jorge E.

AU - Harhaj, Ed

AU - Flavell, Richard A.

N1 - Funding Information: We thank F. Manzo for assistance with manuscript preparation, L. Evangelisti and C. Hughes for generating ES cells and chimeric mice, respectively, J. Stein for initial screening of tiger mice by Southern, and E. Eynon for managing the mouse program. We thank A. Iwasaki for help with isolation of Peyer's patch DC. This work was supported by NIH AI36529 (R.A.F.). Work in J.E.G.'s laboratory is supported by Public Health Service Grant AI055472. R.A.F. is an investigator of the Howard Hughes Medical Institute.

PY - 2006/12

Y1 - 2006/12

N2 - To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.

AB - To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.

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Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger Mouse

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