TY - JOUR
T1 - Expression profiles of Th17 pathway related genes in human systemic lupus erythematosus
AU - Pan, Hai Feng
AU - Leng, Rui Xue
AU - Feng, Chen Chen
AU - Li, Xiang Pei
AU - Chen, Gui Mei
AU - Li, Bao Zhu
AU - Xu, Wang Dong
AU - Zheng, Song Guo
AU - Ye, Dong Qing
N1 - Funding Information:
Acknowledgments This work was supported by grants from the National Natural Science Foundation of China (81102192, 30830089), the Specialized Research Fund for the Doctoral Program
Funding Information:
of Higher Education of China (20113420120008) and the Grants for Scientific Research of BSKY (No. XJ201014) from Anhui Medical University. We thank all the study subjects for their participation.
PY - 2013/1
Y1 - 2013/1
N2 - Recently, evidence is emerging that inappropriate regulation of type 17 T helper cells (Th17) plays a fundamental role in the development of many autoimmune diseases including systemic lupus erythematosus (SLE). However, the role of Th17-related cytokines in SLE remains elusive. To further investigate the role and imbalance of Th17-related cytokines in the pathogenesis of SLE. A Quantitative RT-PCR Array (Human Th17 for Autoimmunity & Inflammation PCR Array) analyses were performed to study Th17-related genes expression in peripheral white blood cells of 25 new-onset patients with SLE and 15 healthy subjects. When gene expression for SLE patients was compared to the mean of normal controls, among the 84 target genes related to Th17 pathway, 7 (CXCL1, ICAM1, IL10, IL5, IL8, ISG20, JAK2,) were upregulated and 6 (CD28, CD40LG, S1PR1, IL17RE, IL23R, RORC) downregulated. However, comparisons of mRNA expression of Th17 related cytokines between lupus nephritis (LN) patients and SLE patients without nephritis (SLE non LN) showed no significant difference. In conclusion, SLE patients and normal controls showed different expression of a few genes in Th17 pathway, indicating that the pathway may be involved in the pathogenesis of SLE.
AB - Recently, evidence is emerging that inappropriate regulation of type 17 T helper cells (Th17) plays a fundamental role in the development of many autoimmune diseases including systemic lupus erythematosus (SLE). However, the role of Th17-related cytokines in SLE remains elusive. To further investigate the role and imbalance of Th17-related cytokines in the pathogenesis of SLE. A Quantitative RT-PCR Array (Human Th17 for Autoimmunity & Inflammation PCR Array) analyses were performed to study Th17-related genes expression in peripheral white blood cells of 25 new-onset patients with SLE and 15 healthy subjects. When gene expression for SLE patients was compared to the mean of normal controls, among the 84 target genes related to Th17 pathway, 7 (CXCL1, ICAM1, IL10, IL5, IL8, ISG20, JAK2,) were upregulated and 6 (CD28, CD40LG, S1PR1, IL17RE, IL23R, RORC) downregulated. However, comparisons of mRNA expression of Th17 related cytokines between lupus nephritis (LN) patients and SLE patients without nephritis (SLE non LN) showed no significant difference. In conclusion, SLE patients and normal controls showed different expression of a few genes in Th17 pathway, indicating that the pathway may be involved in the pathogenesis of SLE.
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U2 - 10.1007/s11033-012-2073-2
DO - 10.1007/s11033-012-2073-2
M3 - Article
C2 - 23054011
AN - SCOPUS:84871326281
SN - 0301-4851
VL - 40
SP - 391
EP - 399
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 1
ER -
