Extracting structural insights from soft X-ray scattering of biological assemblies

Sintu Rongpipi, Joshua T. Del Mundo, Enrique D. Gomez, Esther W. Gomez

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Resonant soft X-ray scattering (RSoXS), a technique that combines X-ray absorption spectroscopy and X-ray scattering, can probe the nano- and meso-scale structure of biological assemblies with chemical specificity. RSoXS experiments yield scattering data collected at several photon energies, for example across an elemental absorption edge of interest. Collecting a near-edge X-ray absorption fine structure (NEXAFS) spectrum complements RSoXS experiments and determines X-ray energies that are best suited for RSoXS measurements. The analysis of RSoXS data is similar in many ways to analysis of small angle X-ray scattering using hard X-rays, with an added dimension that includes an X-ray energy dependence. This chapter discusses procedures for predicting scattering contrast and thereby identifying energies suitable for RSoXS measurements using NEXAFS spectra, analyses of 2D RSoXS images through integration into 1D profiles, and strategies for elucidating the origin of RSoXS scattering features. It also discusses existing and potential methods for interpretation of RSoXS data to gain detailed structural insights into biological systems.

Original languageEnglish (US)
Title of host publicationSmall Angle Scattering Part B
Subtitle of host publicationMethods for Structural Interpretation
EditorsJohn A. Tainer
PublisherAcademic Press Inc.
Number of pages24
ISBN (Print)9780323991810
StatePublished - Jan 2023

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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