Factors influencing regeneration of soybean from mature and immature cotyledons

G. Franklin; L. Carpenter; E. Davis; C. S. Reddy; D. Al-Abed; W. Abou Alaiwi; M. Parani; B. Smith; R. V. Sairam

doi:10.1023/B:GROW.0000038359.86756.18

Factors influencing regeneration of soybean from mature and immature cotyledons

G. Franklin, L. Carpenter, E. Davis, C. S. Reddy, D. Al-Abed, W. Abou Alaiwi, M. Parani, B. Smith, R. V. Sairam

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Soybean (Glycine max L.) plantlets were e?ciently regenerated from mature and immature cotyledons of ?ve different genotypes by studying various parameters affecting regeneration. Green organogenic nodules were induced at the proximal end, which subsequently differentiated into shoot buds on modi?ed MS (Murashige T. and Skoog F. 1962. Physiol. Plant. 15: 473-497) medium. The presence of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the medium exerted a synergistic effect, in that regeneration effciency was higher than for either cytokin alone. The regenerated shoot buds elongated and rooted on MS medium containing 0.29 lM gibberellic acid (GA3) and 2.69 lM a-naphthaleneaceticacid (NAA), respectively. Rooted plants were established in the greenhouse with 87% success and produced viable seeds. Preliminary studies with Agrobacterium show great promise for soybean transformation based on the regeneration protocol reported here.

Original language	English (US)
Pages (from-to)	73-79
Number of pages	7
Journal	Plant Growth Regulation
Volume	43
Issue number	1
DOIs	https://doi.org/10.1023/B:GROW.0000038359.86756.18
State	Published - May 2004

All Science Journal Classification (ASJC) codes

Physiology
Agronomy and Crop Science
Plant Science

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10.1023/B:GROW.0000038359.86756.18

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title = "Factors influencing regeneration of soybean from mature and immature cotyledons",

abstract = "Soybean (Glycine max L.) plantlets were e?ciently regenerated from mature and immature cotyledons of ?ve different genotypes by studying various parameters affecting regeneration. Green organogenic nodules were induced at the proximal end, which subsequently differentiated into shoot buds on modi?ed MS (Murashige T. and Skoog F. 1962. Physiol. Plant. 15: 473-497) medium. The presence of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the medium exerted a synergistic effect, in that regeneration effciency was higher than for either cytokin alone. The regenerated shoot buds elongated and rooted on MS medium containing 0.29 lM gibberellic acid (GA3) and 2.69 lM a-naphthaleneaceticacid (NAA), respectively. Rooted plants were established in the greenhouse with 87% success and produced viable seeds. Preliminary studies with Agrobacterium show great promise for soybean transformation based on the regeneration protocol reported here.",

author = "G. Franklin and L. Carpenter and E. Davis and Reddy, {C. S.} and D. Al-Abed and {Abou Alaiwi}, W. and M. Parani and B. Smith and Sairam, {R. V.}",

note = "Funding Information: We thank A. Hampton, B. Shadia, L.M. Saleena and L. Delp for their technical help. The research was supported by United States Department of",

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AU - Franklin, G.

AU - Carpenter, L.

AU - Davis, E.

AU - Reddy, C. S.

AU - Al-Abed, D.

AU - Abou Alaiwi, W.

AU - Parani, M.

AU - Smith, B.

AU - Sairam, R. V.

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PY - 2004/5

Y1 - 2004/5

N2 - Soybean (Glycine max L.) plantlets were e?ciently regenerated from mature and immature cotyledons of ?ve different genotypes by studying various parameters affecting regeneration. Green organogenic nodules were induced at the proximal end, which subsequently differentiated into shoot buds on modi?ed MS (Murashige T. and Skoog F. 1962. Physiol. Plant. 15: 473-497) medium. The presence of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the medium exerted a synergistic effect, in that regeneration effciency was higher than for either cytokin alone. The regenerated shoot buds elongated and rooted on MS medium containing 0.29 lM gibberellic acid (GA3) and 2.69 lM a-naphthaleneaceticacid (NAA), respectively. Rooted plants were established in the greenhouse with 87% success and produced viable seeds. Preliminary studies with Agrobacterium show great promise for soybean transformation based on the regeneration protocol reported here.

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Factors influencing regeneration of soybean from mature and immature cotyledons

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