Factors influencing regeneration of soybean from mature and immature cotyledons

G. Franklin, L. Carpenter, E. Davis, C. S. Reddy, D. Al-Abed, W. Abou Alaiwi, M. Parani, B. Smith, R. V. Sairam

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33 Scopus citations


Soybean (Glycine max L.) plantlets were e?ciently regenerated from mature and immature cotyledons of ?ve different genotypes by studying various parameters affecting regeneration. Green organogenic nodules were induced at the proximal end, which subsequently differentiated into shoot buds on modi?ed MS (Murashige T. and Skoog F. 1962. Physiol. Plant. 15: 473-497) medium. The presence of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the medium exerted a synergistic effect, in that regeneration effciency was higher than for either cytokin alone. The regenerated shoot buds elongated and rooted on MS medium containing 0.29 lM gibberellic acid (GA3) and 2.69 lM a-naphthaleneaceticacid (NAA), respectively. Rooted plants were established in the greenhouse with 87% success and produced viable seeds. Preliminary studies with Agrobacterium show great promise for soybean transformation based on the regeneration protocol reported here.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalPlant Growth Regulation
Issue number1
StatePublished - May 2004

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science


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