False positive fecal coliform in biosolid samples assayed using a-1 medium

Katherine H. Baker; Brady Redmond; Diane S. Herson

doi:10.2175/106143005X52166

False positive fecal coliform in biosolid samples assayed using a-1 medium

Katherine H. Baker, Brady Redmond, Diane S. Herson

School of Science, Engineering & Technology (Harrisburg)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)-were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.

Original language	English (US)
Pages (from-to)	423-424
Number of pages	2
Journal	Water Environment Research
Volume	77
Issue number	4
DOIs	https://doi.org/10.2175/106143005X52166
State	Published - 2005

All Science Journal Classification (ASJC) codes

Environmental Chemistry
Ecological Modeling
Water Science and Technology
Waste Management and Disposal
Pollution

Access to Document

10.2175/106143005X52166

Cite this

@article{52ab657609514bd29062728b5f2efa3b,

title = "False positive fecal coliform in biosolid samples assayed using a-1 medium",

abstract = "Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)-were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.",

author = "Baker, {Katherine H.} and Brady Redmond and Herson, {Diane S.}",

year = "2005",

doi = "10.2175/106143005X52166",

language = "English (US)",

volume = "77",

pages = "423--424",

journal = "Water Environment Research",

issn = "1061-4303",

publisher = "Water Environment Federation",

number = "4",

}

TY - JOUR

T1 - False positive fecal coliform in biosolid samples assayed using a-1 medium

AU - Baker, Katherine H.

AU - Redmond, Brady

AU - Herson, Diane S.

PY - 2005

Y1 - 2005

N2 - Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)-were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.

AB - Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)-were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.

UR - http://www.scopus.com/inward/record.url?scp=22244489675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22244489675&partnerID=8YFLogxK

U2 - 10.2175/106143005X52166

DO - 10.2175/106143005X52166

M3 - Article

C2 - 16121511

AN - SCOPUS:22244489675

SN - 1061-4303

VL - 77

SP - 423

EP - 424

JO - Water Environment Research

JF - Water Environment Research

IS - 4

ER -

False positive fecal coliform in biosolid samples assayed using a-1 medium

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this