TY - JOUR
T1 - Fatal atypical O:3 Yersinia pseudotuberculosis infection in cynomolgus macaques
AU - Zao, Chih Ling
AU - Tomanek, Lisa
AU - Hurtado-McClure, Graciela
AU - Cooke, Anthony
AU - Berger, Ron
AU - Boulineau, Theresa M.
AU - Turner, Oliver C.
AU - Covington, Danielle E.
N1 - Funding Information:
We thank Claudia Crandall, Kimmi Schrader, Martha Obeso and Dr. Rita Brenden for their assistances with the serotyping experiment in California Department of Public Health. We also thank Patty Srisawangvong for her help in PCR, and Joanne Starkey for her assistance in manuscript preparation. Lastly, we thank the Laboratory Animal Services and the Pathology departments at Novartis Pharmaceuticals for assisting with sample collection and processing. This work was supported by VRL Laboratories and Novartis Pharmaceuticals Corporation .
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/10/25
Y1 - 2013/10/25
N2 - Fatal Yersinia pseudotuberculosis infection in cynomolgus macaques was diagnosed based upon pathology, microbiology and PCR for this study. Pathological findings included acute, erosive to ulcerative, necrohemorrhagic enterocolitis. Genotyping by PCR showed an O:3 pattern (gmd-fcl+, ddhC-prt+, manB+, ddhA-B+), but an additional gene, wbyK, was detected. This is the second report to identify wbyK+ O:3 genotype as the cause of fatal yersiniosis. The first case was reported in 2008, and involved farm deer in the U.S. As the frequency of wbyK+ O:3 genotype is found more often in different carriers, O:3 genotype is proposed to be divided into two subtypes: O:3a without wbyK and O:3b with wbyK. Virulence gene analysis showed the presence of inv, ypmC, irp1, ybtP-ybtQ, yadA, yopB, yopH, lcrF, and suggested that this O:3b isolate could be a highly pathogenic strain to cynomolgus macaques.
AB - Fatal Yersinia pseudotuberculosis infection in cynomolgus macaques was diagnosed based upon pathology, microbiology and PCR for this study. Pathological findings included acute, erosive to ulcerative, necrohemorrhagic enterocolitis. Genotyping by PCR showed an O:3 pattern (gmd-fcl+, ddhC-prt+, manB+, ddhA-B+), but an additional gene, wbyK, was detected. This is the second report to identify wbyK+ O:3 genotype as the cause of fatal yersiniosis. The first case was reported in 2008, and involved farm deer in the U.S. As the frequency of wbyK+ O:3 genotype is found more often in different carriers, O:3 genotype is proposed to be divided into two subtypes: O:3a without wbyK and O:3b with wbyK. Virulence gene analysis showed the presence of inv, ypmC, irp1, ybtP-ybtQ, yadA, yopB, yopH, lcrF, and suggested that this O:3b isolate could be a highly pathogenic strain to cynomolgus macaques.
UR - https://www.scopus.com/pages/publications/84883300681
UR - https://www.scopus.com/pages/publications/84883300681#tab=citedBy
U2 - 10.1016/j.vetmic.2013.07.013
DO - 10.1016/j.vetmic.2013.07.013
M3 - Article
C2 - 23932075
AN - SCOPUS:84883300681
SN - 0378-1135
VL - 166
SP - 681
EP - 685
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -