TY - JOUR
T1 - Fish oil alters tamoxifen-modulated expression of mRNAs that encode genes related to differentiation, proliferation, metastasis, and immune response in rat mammary tumors
AU - Bidinotto, Lucas Tadeu
AU - De Cicco, Ricardo López
AU - Vanegas, Johana Erika
AU - Santucci-Pereira, Julia
AU - Heuvel, John Patrick Vanden
AU - Washington, Sharlene
AU - Aliaga, Cesar
AU - Xu, Haifang
AU - Russo, Irma H.
AU - Manni, Andrea
AU - El-Bayoumy, Karam
AU - Russo, Jose
N1 - Funding Information:
L.T. Bidinotto was the recipient of a 1-year fellowship PDEE (CAPES/Brazil, 067509-1). The support and advice of Dr. Luis Fernando Barbisan are greatly appreciated. We thank Daniel Hannon for his technical support. This project was supported by the Komen Grant (KG081632).
PY - 2012/10/1
Y1 - 2012/10/1
N2 - We have previously shown that a fish oil (FO)-rich diet increased the chemopreventive efficacy of tamoxifen (Tam) against N-methyl-N-nitrosourea (MNU)-induced rat mammary carcinogenesis. Herein, we provide evidence that Tam treatment modifies gene expression of mammary tumors depending upon the type of dietary fat fed to the animals. Rats initiated with MNU and treated with Tam were fed a diet rich in corn oil or FO. After 8 wk, cribriform tumors were collected and gene expression analysis was performed. Increased RNA expression of genes such as SerpinB10, Wisp2, and Apod in tumors from FO-treated rats is indicative of highly differentiated tumors. Decreased expression of H19 and Igf2 mRNA in Tam-treated groups, and Gamma Synuclein mRNA in the FO + Tam group may be related to tumor growth impairment and lower metastatic capacity. Change in the expression of genes associated with immunity in animals in the FO + Tam group may suggest a shift in the immune response. These data show that, although Tam modulates the expression of genes leading to tumor growth impairment, further modulations of genes are influenced by FO. FO modulation of Tam changes in gene expression accounts for its enhancing chemopreventive effect against MNU-induced mammary carcinogenesis. Supplemental materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer to view the supplemental file.
AB - We have previously shown that a fish oil (FO)-rich diet increased the chemopreventive efficacy of tamoxifen (Tam) against N-methyl-N-nitrosourea (MNU)-induced rat mammary carcinogenesis. Herein, we provide evidence that Tam treatment modifies gene expression of mammary tumors depending upon the type of dietary fat fed to the animals. Rats initiated with MNU and treated with Tam were fed a diet rich in corn oil or FO. After 8 wk, cribriform tumors were collected and gene expression analysis was performed. Increased RNA expression of genes such as SerpinB10, Wisp2, and Apod in tumors from FO-treated rats is indicative of highly differentiated tumors. Decreased expression of H19 and Igf2 mRNA in Tam-treated groups, and Gamma Synuclein mRNA in the FO + Tam group may be related to tumor growth impairment and lower metastatic capacity. Change in the expression of genes associated with immunity in animals in the FO + Tam group may suggest a shift in the immune response. These data show that, although Tam modulates the expression of genes leading to tumor growth impairment, further modulations of genes are influenced by FO. FO modulation of Tam changes in gene expression accounts for its enhancing chemopreventive effect against MNU-induced mammary carcinogenesis. Supplemental materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer to view the supplemental file.
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U2 - 10.1080/01635581.2012.712736
DO - 10.1080/01635581.2012.712736
M3 - Article
C2 - 23061905
AN - SCOPUS:84867833802
SN - 0163-5581
VL - 64
SP - 991
EP - 999
JO - Nutrition and cancer
JF - Nutrition and cancer
IS - 7
ER -