TY - GEN
T1 - Fluorescent labeling, sensing and differentiation of leukocytes from undiluted whole blood samples
AU - Zheng, Siyang
AU - Lin, Jeffrey Chun Hui
AU - Kasdan, Harvey L.
AU - Tai, Yu Chong
N1 - Funding Information:
This work is supported by the National Space Biomedical Research Institute through NASA NCC 9-58. The authors would like to thank other members of the Caltech Micromachining Laboratory for their valuable assistance.
Funding Information:
Yu-Chong Tai received his BS degree from National Taiwan University, and the MS and PhD degrees in Electrical Engineering from the University of California at Berkeley. After Berkeley, he joined the faculty of Electrical Engineering at the California Institute of Technology and built the Caltech MEMS Lab. Not long ago, he joined the Bioengineering department and he is currently a Professor of Electrical Engineering and Bioengineering at Caltech. His current research interests include flexible MEMS, bioMEMS, MEMS for retinal implants, parylene-based integrated microfluidics, neuroprobes/neurochips, HPLC-based labs-on-a-chip. He has received several awards such as the IBM fellowship, the Best Thesis Award, the Presidential Young Investigator (PYI) Award and the David and Lucile Packard Fellowship. He co-chaired the 2002 IEEE MEMS Conference in Las Vegas. He is currently a Subject Editor of the Journal of Microelectromechanical Systems.
PY - 2007
Y1 - 2007
N2 - In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices. A challenging issue in leukocyte sensing from blood samples is the required high dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and processing time, but also requires mixing and buffer storage for on-chip implementation. Unlike commercial bulk instruments and previous efforts by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye Acridine Orange (AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525nm was used for leukocyte count and red fluorescent signal centered at 650nm was used for leukocyte differentiation. Throughput of one hundred leukocytes per second was achieved, which means operation time for one sample only requires several seconds.
AB - In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices. A challenging issue in leukocyte sensing from blood samples is the required high dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and processing time, but also requires mixing and buffer storage for on-chip implementation. Unlike commercial bulk instruments and previous efforts by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye Acridine Orange (AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525nm was used for leukocyte count and red fluorescent signal centered at 650nm was used for leukocyte differentiation. Throughput of one hundred leukocytes per second was achieved, which means operation time for one sample only requires several seconds.
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U2 - 10.1109/SENSOR.2007.4300253
DO - 10.1109/SENSOR.2007.4300253
M3 - Conference contribution
AN - SCOPUS:50049105019
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 807
EP - 810
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Y2 - 10 June 2007 through 14 June 2007
ER -