TY - JOUR
T1 - Fluorescent labeling, sensing, and differentiation of leukocytes from undiluted whole blood samples
AU - Zheng, Siyang
AU - Lin, Jeffrey Chun Hui
AU - Kasdan, Harvey L.
AU - Tai, Yu Chong
N1 - Funding Information:
Yu-Chong Tai received his BS degree from National Taiwan University, and the MS and PhD degrees in Electrical Engineering from the University of California at Berkeley. After Berkeley, he joined the faculty of Electrical Engineering at the California Institute of Technology and built the Caltech MEMS Lab. Not long ago, he joined the Bioengineering department and he is currently a Professor of Electrical Engineering and Bioengineering at Caltech. His current research interests include flexible MEMS, bioMEMS, MEMS for retinal implants, parylene-based integrated microfluidics, neuroprobes/neurochips, HPLC-based labs-on-a-chip. He has received several awards such as the IBM fellowship, the Best Thesis Award, the Presidential Young Investigator (PYI) Award and the David and Lucile Packard Fellowship. He co-chaired the 2002 IEEE MEMS Conference in Las Vegas. He is currently a Subject Editor of the Journal of Microelectromechanical Systems.
Funding Information:
This work is supported by the National Space Biomedical Research Institute through NASA NCC 9-58. The authors would like to thank other members of the Caltech Micromachining Laboratory for their valuable assistance.
PY - 2008/6/16
Y1 - 2008/6/16
N2 - In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices. A challenging issue in leukocyte sensing from blood samples is the required high-dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and processing time, but also requires mixing and buffer storage for on-chip implementation. Unlike commercial bulk instruments and previous efforts by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye acridine orange (AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525 nm is used for leukocyte count and red fluorescent signal centered at 650 nm is used for leukocyte differentiation. Throughput of 1000 leukocytes per second was achieved.
AB - In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices. A challenging issue in leukocyte sensing from blood samples is the required high-dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and processing time, but also requires mixing and buffer storage for on-chip implementation. Unlike commercial bulk instruments and previous efforts by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye acridine orange (AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525 nm is used for leukocyte count and red fluorescent signal centered at 650 nm is used for leukocyte differentiation. Throughput of 1000 leukocytes per second was achieved.
UR - http://www.scopus.com/inward/record.url?scp=44649161186&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44649161186&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2007.11.031
DO - 10.1016/j.snb.2007.11.031
M3 - Article
AN - SCOPUS:44649161186
SN - 0925-4005
VL - 132
SP - 558
EP - 567
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 2
ER -