TY - GEN
T1 - Fortuna Detects Novel Splicing in Drosophila scRNASeq Data
AU - Borozan, B.
AU - Borozan, L.
AU - Severdija, D.
AU - Matijevic, D.
AU - Canzar, S.
N1 - Publisher Copyright:
© 2023 MIPRO Croatian Society.
PY - 2023
Y1 - 2023
N2 - Recent developments in single-cell RNA sequencing techniques (scRNASeq) have made large quantities of sequenced data available across numerous species and tissues. Alternative splicing (AS) of pre-mRNA introns varies between tissues and even between cell-types and can be altered in disease. The study of novel AS, using standard RNASeq data, has been extensively studied for many years, while similar work on scRNASeq data has been scarce, despite its potential to offer a broader insight into cell-type specific processes. In this paper, we propose a novel pipeline that uses fortuna, a method that efficiently classifies and quantifies novel AS events, to process scRNASeq samples. Due to its short lifespan, high number of progeny, low maintenance cost, and intricate alternative splicing patterns similar in complexity to those of mammals, Drosophila Melanogaster (fruit fly) is a species of particular interest to researchers. Therefore, we experimentally evaluate our pipeline on real-world Drosophila single-cell data samples from the Fly Cell Atlas.
AB - Recent developments in single-cell RNA sequencing techniques (scRNASeq) have made large quantities of sequenced data available across numerous species and tissues. Alternative splicing (AS) of pre-mRNA introns varies between tissues and even between cell-types and can be altered in disease. The study of novel AS, using standard RNASeq data, has been extensively studied for many years, while similar work on scRNASeq data has been scarce, despite its potential to offer a broader insight into cell-type specific processes. In this paper, we propose a novel pipeline that uses fortuna, a method that efficiently classifies and quantifies novel AS events, to process scRNASeq samples. Due to its short lifespan, high number of progeny, low maintenance cost, and intricate alternative splicing patterns similar in complexity to those of mammals, Drosophila Melanogaster (fruit fly) is a species of particular interest to researchers. Therefore, we experimentally evaluate our pipeline on real-world Drosophila single-cell data samples from the Fly Cell Atlas.
UR - http://www.scopus.com/inward/record.url?scp=85164954147&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85164954147&partnerID=8YFLogxK
U2 - 10.23919/MIPRO57284.2023.10159716
DO - 10.23919/MIPRO57284.2023.10159716
M3 - Conference contribution
AN - SCOPUS:85164954147
T3 - 2023 46th ICT and Electronics Convention, MIPRO 2023 - Proceedings
SP - 410
EP - 415
BT - 2023 46th ICT and Electronics Convention, MIPRO 2023 - Proceedings
A2 - Cisic, Dragan
A2 - Vrcek, Neven
A2 - Koricic, Marko
A2 - Gradisnik, Vera
A2 - Skala, Karolj
A2 - Car, Zeljka
A2 - Cicin-Sain, Marina
A2 - Babic, Snjezana
A2 - Sruk, Vlado
A2 - Skvorc, Dejan
A2 - Jovic, Alan
A2 - Gros, Stjepan
A2 - Vrdoljak, Boris
A2 - Tijan, Edvard
A2 - Katulic, Tihomir
A2 - Petrovic, Juraj
A2 - Grbac, Tihana Galinac
A2 - Bozicevic, Lovro
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 46th ICT and Electronics Convention, MIPRO 2023
Y2 - 22 May 2023 through 26 May 2023
ER -