TY - JOUR
T1 - Four products from Escherichia coli pseudogenes increase hydrogen production
AU - Mohd Yusoff, Mohd Zulkhairi
AU - Hashiguchi, Yuya
AU - Maeda, Toshinari
AU - Wood, Thomas K.
N1 - Funding Information:
Special acknowledgement goes to the NBRP- E. coli at the National of Institute of Genetics (Japan) for providing Keio mutants and ASKA clones. In addition, this research was performed as an international collaboration under the Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation (R2217) supported by the Japan Society for the Promotion of Science. In addition, we acknowledge the Japan Science Society through Sasakawa Scientific Research Grant (24-521) and JGC-S scholarship foundation for supporting this research.
PY - 2013/10/4
Y1 - 2013/10/4
N2 - Pseudogenes are considered to be nonfunctional genes that lack a physiological role. By screening 3985 Escherichia coli mutants using chemochromic membranes, we found four pseudogenes involved in hydrogen metabolism. Knockouts of pseudogenes ydfW and ypdJ had a defective hydrogen phenotype on glucose and formate, respectively. Also, the knockout of pseudogene yqiG formed hydrogen from formate but not from glucose. For the yqiG mutant, 100% hydrogen recovery was obtained by the complementation of YqiG via a plasmid. The knockout of pseudogene ylcE showed hydrogen deficiency in minimal media which suggested that the role of YlcE is associated with cell growth. Hence, the products of these four pseudogenes play an important physiological role in hydrogen production in E. coli.
AB - Pseudogenes are considered to be nonfunctional genes that lack a physiological role. By screening 3985 Escherichia coli mutants using chemochromic membranes, we found four pseudogenes involved in hydrogen metabolism. Knockouts of pseudogenes ydfW and ypdJ had a defective hydrogen phenotype on glucose and formate, respectively. Also, the knockout of pseudogene yqiG formed hydrogen from formate but not from glucose. For the yqiG mutant, 100% hydrogen recovery was obtained by the complementation of YqiG via a plasmid. The knockout of pseudogene ylcE showed hydrogen deficiency in minimal media which suggested that the role of YlcE is associated with cell growth. Hence, the products of these four pseudogenes play an important physiological role in hydrogen production in E. coli.
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U2 - 10.1016/j.bbrc.2013.09.016
DO - 10.1016/j.bbrc.2013.09.016
M3 - Article
C2 - 24025676
AN - SCOPUS:84884700320
SN - 0006-291X
VL - 439
SP - 576
EP - 579
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -