TY - JOUR
T1 - Freshwater vertebrate metabarcoding on Illumina platforms using double-indexed primers of the mitochondrial 16S rRNA gene
AU - Vences, Miguel
AU - Lyra, Mariana L.
AU - Perl, R. G.Bina
AU - Bletz, Molly C.
AU - Stanković, David
AU - Lopes, Carla Martins
AU - Jarek, Michael
AU - Bhuju, Sabin
AU - Geffers, Robert
AU - Haddad, Célio F.B.
AU - Steinfartz, Sebastian
N1 - Publisher Copyright:
© 2016, Springer Science+Business Media Dordrecht.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Metabarcoding is a promising tool for biodiversity inventories and other applications in conservation genetics. We developed a new pair of primers for efficient and affordable high-throughput analysis of a 250 base pair stretch of DNA from the mitochondrial 16S rRNA gene of vertebrates, especially amphibians and fishes. By adapting a double-indexed protocol for Illumina platforms, our approach allows pooling of hundreds of samples in a single sequencing run. We obtained high detection rates of 82–93 % for fish in two German streams, 70 % for mock mixes of DNA from amphibians and fishes, and could distinguish multiple gene copies in amphibians, probably caused by nuclear-mitochondrial transposed DNA or heteroplasmy.
AB - Metabarcoding is a promising tool for biodiversity inventories and other applications in conservation genetics. We developed a new pair of primers for efficient and affordable high-throughput analysis of a 250 base pair stretch of DNA from the mitochondrial 16S rRNA gene of vertebrates, especially amphibians and fishes. By adapting a double-indexed protocol for Illumina platforms, our approach allows pooling of hundreds of samples in a single sequencing run. We obtained high detection rates of 82–93 % for fish in two German streams, 70 % for mock mixes of DNA from amphibians and fishes, and could distinguish multiple gene copies in amphibians, probably caused by nuclear-mitochondrial transposed DNA or heteroplasmy.
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U2 - 10.1007/s12686-016-0550-y
DO - 10.1007/s12686-016-0550-y
M3 - Article
AN - SCOPUS:84983517731
SN - 1877-7252
VL - 8
SP - 323
EP - 327
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -