TY - JOUR
T1 - Functional and Evolutionary Insights into Human Brain Development through Global Transcriptome Analysis
AU - Johnson, Matthew B.
AU - Imamura, Yuka
AU - Mason, Christopher E.
AU - Krsnik, Željka
AU - Coppola, Giovanni
AU - Bogdanović, Darko
AU - Geschwind, Daniel H.
AU - Mane, Shrikant M.
AU - State, Matthew W.
AU - Šestan, Nenad
N1 - Funding Information:
We thank Bradford Poulos for assistance with tissue acquisition, Aiping Lin for help with microarray platform comparisons, James Noonan for advice on analysis of haCNSs, Fuying Gao for statistical analysis, Donna Karolchik and Andy Pohl for assistance in creating tracks for the Genome Browser, and many colleagues for their help and comments. This work was supported by NIH grants NS054273, MH081896 (to N.S.), NS056276 (to M.W.S.), NS051869 (to S.M.M.), MH060233 (to D.H.G.), Kavli Foundation, NARSAD, and the James S. McDonnell Foundation Scholar Award (to N.S.).
PY - 2009/5/28
Y1 - 2009/5/28
N2 - Our understanding of the evolution, formation, and pathological disruption of human brain circuits is impeded by a lack of comprehensive data on the developing brain transcriptome. A whole-genome, exon-level expression analysis of 13 regions from left and right sides of the mid-fetal human brain revealed that 76% of genes are expressed, and 44% of these are differentially regulated. These data reveal a large number of specific gene expression and alternative splicing patterns, as well as coexpression networks, associated with distinct regions and neurodevelopmental processes. Of particular relevance to cognitive specializations, we have characterized the transcriptional landscapes of prefrontal cortex and perisylvian speech and language areas, which exhibit a population-level global expression symmetry. We show that differentially expressed genes are more frequently associated with human-specific evolution of putative cis-regulatory elements. These data provide a wealth of biological insights into the complex transcriptional and molecular underpinnings of human brain development and evolution.
AB - Our understanding of the evolution, formation, and pathological disruption of human brain circuits is impeded by a lack of comprehensive data on the developing brain transcriptome. A whole-genome, exon-level expression analysis of 13 regions from left and right sides of the mid-fetal human brain revealed that 76% of genes are expressed, and 44% of these are differentially regulated. These data reveal a large number of specific gene expression and alternative splicing patterns, as well as coexpression networks, associated with distinct regions and neurodevelopmental processes. Of particular relevance to cognitive specializations, we have characterized the transcriptional landscapes of prefrontal cortex and perisylvian speech and language areas, which exhibit a population-level global expression symmetry. We show that differentially expressed genes are more frequently associated with human-specific evolution of putative cis-regulatory elements. These data provide a wealth of biological insights into the complex transcriptional and molecular underpinnings of human brain development and evolution.
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U2 - 10.1016/j.neuron.2009.03.027
DO - 10.1016/j.neuron.2009.03.027
M3 - Article
C2 - 19477152
AN - SCOPUS:65649112466
SN - 0896-6273
VL - 62
SP - 494
EP - 509
JO - Neuron
JF - Neuron
IS - 4
ER -