TY - JOUR
T1 - Functional and molecular features of the Id4+ germline stem cell population in mouse testes
AU - Chan, Frieda
AU - Oatley, Melissa J.
AU - Kaucher, Amy V.
AU - Yang, Qi En
AU - Bieberich, Charles J.
AU - Shashikant, Cooduvalli S.
AU - Oatley, Jon M.
PY - 2014/6/15
Y1 - 2014/6/15
N2 - The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermato-genesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ~ 2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp+/stem cell and Id4-Gfp-/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline.
AB - The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermato-genesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ~ 2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp+/stem cell and Id4-Gfp-/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline.
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U2 - 10.1101/gad.240465.114
DO - 10.1101/gad.240465.114
M3 - Article
C2 - 24939937
AN - SCOPUS:84902496118
SN - 0890-9369
VL - 28
SP - 1351
EP - 1362
JO - Genes and Development
JF - Genes and Development
IS - 12
ER -