Functional Role of a Mobile Loop of Escherichia coli Dihydrofolate Reductase in Transition-State Stabilization

Keyphrases

• Functional Role 100%
• In the Wild 100%
• Escherichia Coli Dihydrofolate Reductase 100%
• Transition State Stabilization 100%
• Nicotinamide 66%
• NMR Spectra 66%
• NADP+ 66%
• Hydride Transfer 66%
• Secondary Structure 33%
• Two Dimensional 33%
• Steady State 33%
• Substrate Binding 33%
• Deletion mutant 33%
• Apoprotein 33%
• Glycine 33%
• Slow Process 33%
• X-ray Crystal Structure 33%
• Hairpin 33%
• Exchange Processes 33%
• Folate 33%
• Nicotinamide Adenine Dinucleotide Phosphate (NADPH) 33%
• Binary Complexes 33%
• Ternary Complex 33%
• Transfer Rate 33%
• Substrate Flux 33%
• Hydrophobic Residues 33%
• Steady-state Kinetics 33%
• Transfer Process 33%
• Kinetic Behavior 33%
• Mutant Enzyme 33%
• Two-dimensional NMR 33%
• Rate Decline 33%
• Tetrahydrofolate 33%
• Restricted Movement 33%
• Hairpin Turn 33%

Chemistry

• Transition State 100%
• NMR Spectrum 100%
• Dihydrofolates 100%
• Nicotinamide Adenine Dinucleotide Phosphate 100%
• Pteridine 100%
• Secondary Structure 50%
• NMR Spectroscopy 50%
• pKa 50%
• Rate Constant 50%
• Crystal Structure 50%
• Apoprotein 50%
• Two-Dimensional NMR 50%
• Transfer Process 50%
• NADPH 50%
• Tetrahydrofolate 50%
• Folate 50%
• Tetrahydrofolic Acid 50%

Biochemistry, Genetics and Molecular Biology

• Escherichia coli 100%
• Wild Type 100%
• Dihydrofolate Reductase 100%
• Enzyme 42%
• Steady State 28%
• Nicotinamide Adenine Dinucleotide Phosphate 28%
• Pinnipedia 14%
• Protein Secondary Structure 14%
• Dynamics 14%
• Crystal Structure 14%
• Biochemistry 14%
• Deletion Mutant 14%
• X Ray 14%
• Ternary Complex 14%
• Folic Acid 14%