TY - JOUR
T1 - Functional stability of a mixed microbial consortium producing PHA from waste carbon sources
AU - Coats, Erik R.
AU - Loge, Frank J.
AU - Smith, William A.
AU - Thompson, David N.
AU - Wolcott, Michael P.
N1 - Funding Information:
This material is based on work supported by the National Science Foundation under Grant Number DMI-0400337 and by the U.S. Department of Energy, Industrial Technologies Program, Forest Products Industries of the Future, under DOE-NE Idaho Operations Office Contract DE-AC07-05ID14517. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the funding agency.
PY - 2007/4
Y1 - 2007/4
N2 - Polyhydroxyalkanoates (PHAs) represent an environmentally effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85, 53, and 10% of the cell dry weight from methanol-enriched pulp and paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Using denaturing gradient gel electrophoresis of 16S-rDNA from polymerase chain reaction-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment. Most importantly, functional stability was maintained despite differing and contrasting microbial populations.
AB - Polyhydroxyalkanoates (PHAs) represent an environmentally effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85, 53, and 10% of the cell dry weight from methanol-enriched pulp and paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Using denaturing gradient gel electrophoresis of 16S-rDNA from polymerase chain reaction-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment. Most importantly, functional stability was maintained despite differing and contrasting microbial populations.
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U2 - 10.1007/s12010-007-9107-6
DO - 10.1007/s12010-007-9107-6
M3 - Article
C2 - 18478444
AN - SCOPUS:45249096534
SN - 0273-2289
VL - 137-140
SP - 909
EP - 925
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 1-12
ER -