Fusarium oxysporum infection assays in arabidopsis

Yuri Trusov, David Chakravorty, Jose Ramon Botella

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations


Increased susceptibility to Fusarium oxysporum is one of the most conspicuous characteristics of the Arabidopsis mutants lacking the heterotrimeric G protein β and γ1 subunits. The molecular mechanisms placing these G proteins in the plant innate immunity network are yet to be discovered. However, a method to test susceptibility to and disease progression of an important plant pathogen, such as F. oxysporum, is of central importance to many plant defense studies. The optimized protocol presented here allows the routine processing and analysis of symptom progression in young Arabidopsis soil-grown seedlings and yields highly reproducible results.

Original languageEnglish (US)
Title of host publicationG Protein-Coupled Receptor Signaling in Plants
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages6
ISBN (Print)9781627035316
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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