TY - JOUR
T1 - Ganglioside GD3 enhances adherence of botulinum and tetanus neurotoxins to bovine brain synapsin I
AU - Schengrund, Cara Lynne
AU - DasGupta, Bibhuti R.
AU - Ringler, Nancy J.
N1 - Funding Information:
This work was supported in part by Public Health Service Grants AI 23721 to C.-L.S. and NS17742 to B.R.DG. The authors thank Anne Stanley for carrying out the N-terminal amino acid sequence analyses.
PY - 1993/8/20
Y1 - 1993/8/20
N2 - Tetanus toxin (TTx) and botulinum toxin serotype A (BTxA), preincubated with trisialoganglioside GT1b, adhere to proteins present on blots of bovine synaptosomal proteins. Differential solubilization and ammonium sulfate fractionation provided material enriched in two proteins that appeared to be adhered to most strongly by the labeled neurotoxins. After excision of the appropriate bands from blots of electrophoretically separated proteins, N-terminal amino acid sequence analysis permitted identification of the proteins as synapsins Ia and Ib. Comparison of the effectiveness of different gangliosides at enhancing adherence of the neurotoxins to blots of synapsins Ia and Ib indicated that GD3 was most effective.
AB - Tetanus toxin (TTx) and botulinum toxin serotype A (BTxA), preincubated with trisialoganglioside GT1b, adhere to proteins present on blots of bovine synaptosomal proteins. Differential solubilization and ammonium sulfate fractionation provided material enriched in two proteins that appeared to be adhered to most strongly by the labeled neurotoxins. After excision of the appropriate bands from blots of electrophoretically separated proteins, N-terminal amino acid sequence analysis permitted identification of the proteins as synapsins Ia and Ib. Comparison of the effectiveness of different gangliosides at enhancing adherence of the neurotoxins to blots of synapsins Ia and Ib indicated that GD3 was most effective.
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U2 - 10.1016/0304-3940(93)90253-H
DO - 10.1016/0304-3940(93)90253-H
M3 - Article
C2 - 8233089
AN - SCOPUS:0027162386
SN - 0304-3940
VL - 158
SP - 159
EP - 162
JO - Neuroscience letters
JF - Neuroscience letters
IS - 2
ER -