Ganglioside GM1 enhances induction by nerve growth factor of a putative dimer of TrkA

Tahira Farooqui; Teresa Franklin; Dennis K. Pearl; Allan J. Yates

doi:10.1046/j.1471-4159.1997.68062348.x

Ganglioside GM1 enhances induction by nerve growth factor of a putative dimer of TrkA

Tahira Farooqui, Teresa Franklin, Dennis K. Pearl, Allan J. Yates

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

GM1 enhances nerve growth factor (NGF)stimulated neuritogenesis and prevents apoptotic death of PC12 cells; both may be due to enhancement of TrkA dimerization. In this study, we examined the effect of GM1 on NGF- induced TrkA dimerization in Trk-PC12 (6-24) cells. NGF increased tyrosine phosphorylation of the 140-kDa protein (TrkA monomer), and preincubation with GM1 potentiated this effect. Adding the protein cross-linker bis(sulfosuccinimidyl) suberate with NGF resulted in the appearance of two major bands (220 and 330 kDa) when probed with antibodies against TrkA or phosphotyrosine, and GM1 also enhanced this effect. We interpret the 330-kDa band as being a homodimer of TrkA. The identity of the 220-kDa band is still not certain but may consist of a posttranslationally modified form of TrkA. Our results suggest that GM1 is augmenting the effects of NGF on PC12 cells by enhancing the dimerization and activation of the TrkA receptor.

Original language	English (US)
Pages (from-to)	2348-2355
Number of pages	8
Journal	Journal of neurochemistry
Volume	68
Issue number	6
DOIs	https://doi.org/10.1046/j.1471-4159.1997.68062348.x
State	Published - Jun 1997

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience

Access to Document

10.1046/j.1471-4159.1997.68062348.x

Cite this

@article{ee3f93f496104b03971ea53f4cc3dd0c,

title = "Ganglioside GM1 enhances induction by nerve growth factor of a putative dimer of TrkA",

abstract = "GM1 enhances nerve growth factor (NGF)stimulated neuritogenesis and prevents apoptotic death of PC12 cells; both may be due to enhancement of TrkA dimerization. In this study, we examined the effect of GM1 on NGF- induced TrkA dimerization in Trk-PC12 (6-24) cells. NGF increased tyrosine phosphorylation of the 140-kDa protein (TrkA monomer), and preincubation with GM1 potentiated this effect. Adding the protein cross-linker bis(sulfosuccinimidyl) suberate with NGF resulted in the appearance of two major bands (220 and 330 kDa) when probed with antibodies against TrkA or phosphotyrosine, and GM1 also enhanced this effect. We interpret the 330-kDa band as being a homodimer of TrkA. The identity of the 220-kDa band is still not certain but may consist of a posttranslationally modified form of TrkA. Our results suggest that GM1 is augmenting the effects of NGF on PC12 cells by enhancing the dimerization and activation of the TrkA receptor.",

author = "Tahira Farooqui and Teresa Franklin and Pearl, {Dennis K.} and Yates, {Allan J.}",

year = "1997",

month = jun,

doi = "10.1046/j.1471-4159.1997.68062348.x",

language = "English (US)",

volume = "68",

pages = "2348--2355",

journal = "Journal of neurochemistry",

issn = "0022-3042",

publisher = "John Wiley and Sons Inc.",

number = "6",

}

TY - JOUR

T1 - Ganglioside GM1 enhances induction by nerve growth factor of a putative dimer of TrkA

AU - Farooqui, Tahira

AU - Franklin, Teresa

AU - Pearl, Dennis K.

AU - Yates, Allan J.

PY - 1997/6

Y1 - 1997/6

N2 - GM1 enhances nerve growth factor (NGF)stimulated neuritogenesis and prevents apoptotic death of PC12 cells; both may be due to enhancement of TrkA dimerization. In this study, we examined the effect of GM1 on NGF- induced TrkA dimerization in Trk-PC12 (6-24) cells. NGF increased tyrosine phosphorylation of the 140-kDa protein (TrkA monomer), and preincubation with GM1 potentiated this effect. Adding the protein cross-linker bis(sulfosuccinimidyl) suberate with NGF resulted in the appearance of two major bands (220 and 330 kDa) when probed with antibodies against TrkA or phosphotyrosine, and GM1 also enhanced this effect. We interpret the 330-kDa band as being a homodimer of TrkA. The identity of the 220-kDa band is still not certain but may consist of a posttranslationally modified form of TrkA. Our results suggest that GM1 is augmenting the effects of NGF on PC12 cells by enhancing the dimerization and activation of the TrkA receptor.

AB - GM1 enhances nerve growth factor (NGF)stimulated neuritogenesis and prevents apoptotic death of PC12 cells; both may be due to enhancement of TrkA dimerization. In this study, we examined the effect of GM1 on NGF- induced TrkA dimerization in Trk-PC12 (6-24) cells. NGF increased tyrosine phosphorylation of the 140-kDa protein (TrkA monomer), and preincubation with GM1 potentiated this effect. Adding the protein cross-linker bis(sulfosuccinimidyl) suberate with NGF resulted in the appearance of two major bands (220 and 330 kDa) when probed with antibodies against TrkA or phosphotyrosine, and GM1 also enhanced this effect. We interpret the 330-kDa band as being a homodimer of TrkA. The identity of the 220-kDa band is still not certain but may consist of a posttranslationally modified form of TrkA. Our results suggest that GM1 is augmenting the effects of NGF on PC12 cells by enhancing the dimerization and activation of the TrkA receptor.

UR - http://www.scopus.com/inward/record.url?scp=0030929378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030929378&partnerID=8YFLogxK

U2 - 10.1046/j.1471-4159.1997.68062348.x

DO - 10.1046/j.1471-4159.1997.68062348.x

M3 - Article

C2 - 9166727

AN - SCOPUS:0030929378

SN - 0022-3042

VL - 68

SP - 2348

EP - 2355

JO - Journal of neurochemistry

JF - Journal of neurochemistry

IS - 6

ER -

Ganglioside GM1 enhances induction by nerve growth factor of a putative dimer of TrkA

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this