Ganglioside-induced adherence of botulinum and tetanus neurotoxins to adducin

Cara Lynne Schengrund, Bibhuti R. DasGupta, Christine A. Hughes, Nancy J. Ringler

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Preincubation of botulinum neurotoxin serotype A, B, or E with ganglioside GT1b was previously found to enhance adherence of botulinum neurotoxin to synapsin I and an ~116-kDa bovine brain synaptosomal protein; in contrast, adherence to these two proteins by tetanus neurotoxin required preincubation with GT1b. We have now found that preincubation of the neurotoxins with ganglioside GD3 enhances their adherence to the ~116-kDa protein more than that with GT1b. A purified preparation of the water-soluble ~116-kDa protein was obtained from bovine brain synaptosomes by preparative column sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. N-Terminal amino acid sequences were obtained for two tryptic fragments of the ~116-kDa protein. These sequences matched with the data bank sequences for β-adducin, a cytoskeletal protein. The carboxy- terminal tail region of adducin, but not the head region, was adhered to by the neurotoxins. Adherence of the neurotoxin to adducin and synapsin I may facilitate presentation of the neurotoxin to its specific substrate(s).

Original languageEnglish (US)
Pages (from-to)2556-2561
Number of pages6
JournalJournal of neurochemistry
Issue number6
StatePublished - Jun 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience


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