Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata

C. C. Judice; M. F. Carazzole; F. Festa; M. C. Sogayar; K. Hartfelder; G. A.G. Pereira

doi:10.1111/j.1365-2583.2005.00605.x

Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata

C. C. Judice, M. F. Carazzole, F. Festa, M. C. Sogayar, K. Hartfelder, G. A.G. Pereira

Department of Pediatrics

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.

Original language	English (US)
Pages (from-to)	33-44
Number of pages	12
Journal	Insect Molecular Biology
Volume	15
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2583.2005.00605.x
State	Published - Feb 2006

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Insect Science

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10.1111/j.1365-2583.2005.00605.x

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title = "Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata",

abstract = "To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.",

author = "Judice, {C. C.} and Carazzole, {M. F.} and F. Festa and Sogayar, {M. C.} and K. Hartfelder and Pereira, {G. A.G.}",

note = "Funding Information: We thank Losehill Hall for providing the apiary site. J.A. P.S was supported by a Ph.D. scholarship from CONACyT (M{\`e}xico) and Colegio de Postgraduados (M{\`e}xico). M.J.C was supported by a Ph.D. scholarship from the National Science Foundation (USA). W.O.H.H. was supported by a Marie Curie Outgoing International Fellowship within the 6th European Community Framework Programme.",

year = "2006",

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doi = "10.1111/j.1365-2583.2005.00605.x",

language = "English (US)",

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AU - Judice, C. C.

AU - Carazzole, M. F.

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AU - Sogayar, M. C.

AU - Hartfelder, K.

AU - Pereira, G. A.G.

N1 - Funding Information: We thank Losehill Hall for providing the apiary site. J.A. P.S was supported by a Ph.D. scholarship from CONACyT (Mèxico) and Colegio de Postgraduados (Mèxico). M.J.C was supported by a Ph.D. scholarship from the National Science Foundation (USA). W.O.H.H. was supported by a Marie Curie Outgoing International Fellowship within the 6th European Community Framework Programme.

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N2 - To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.

AB - To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.

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Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata

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