TY - JOUR
T1 - Generating fixed concentration arrays in a microfluidic device
AU - Holden, Matthew A.
AU - Kumar, Saurabh
AU - Castellana, Edward T.
AU - Beskok, Ali
AU - Cremer, Paul S.
N1 - Funding Information:
This work was funded by an ONR-YIP Award (NOOO14-00-1-0664) and by ARO (DAAD 19-01-1-0346). We would also like to acknowledge support from the Center for Integrated Microchemical Systems at Texas A&M University and the use of the TAMU/CIMS Materials Characterization Facility. PSC also gratefully acknowledges the receipt of a Beckman Young Investigator Award and a Nontenured Faculty Award from 3M Corporation.
Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - We have designed and built a laminar microfluidic diffusion diluter (μDD) to obtain fixed concentration gradients inside lithographically patterned lab-on-a-chip architectures. The driving force for this investigation was the desire to minimize the amount of precious analyte consumed in high throughput measurements performed as a function of concentration. This was achieved by engineering a microfluidic system capable of delivering minute volumes of analyte by very slow pressure-driven flow. The μDD consists of a Y-junction that allows inflow of two different streams into a main channel, which eventually splits into a linear array of independent microchannels. The arraying technique is based on convective/diffusive transport of nanoliter quantities of an analyte from one fluid stream into the other. The μDD design allows output channels to exhibit predetermined analyte concentration values, which can be controlled by regulating the flow rate. Experiments were performed for flow rates ranging from 500 to 50nl/min. Theoretical studies of convective/diffusive transport in the main channel have been performed as a function of the Peclet number and the normalized channel dimensions. These results were validated using fluorescence microscopy experiments as well as two- and three-dimensional numerical simulations. The computational results compared well with the experimental measurements, validating the μDD design.
AB - We have designed and built a laminar microfluidic diffusion diluter (μDD) to obtain fixed concentration gradients inside lithographically patterned lab-on-a-chip architectures. The driving force for this investigation was the desire to minimize the amount of precious analyte consumed in high throughput measurements performed as a function of concentration. This was achieved by engineering a microfluidic system capable of delivering minute volumes of analyte by very slow pressure-driven flow. The μDD consists of a Y-junction that allows inflow of two different streams into a main channel, which eventually splits into a linear array of independent microchannels. The arraying technique is based on convective/diffusive transport of nanoliter quantities of an analyte from one fluid stream into the other. The μDD design allows output channels to exhibit predetermined analyte concentration values, which can be controlled by regulating the flow rate. Experiments were performed for flow rates ranging from 500 to 50nl/min. Theoretical studies of convective/diffusive transport in the main channel have been performed as a function of the Peclet number and the normalized channel dimensions. These results were validated using fluorescence microscopy experiments as well as two- and three-dimensional numerical simulations. The computational results compared well with the experimental measurements, validating the μDD design.
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U2 - 10.1016/S0925-4005(03)00129-1
DO - 10.1016/S0925-4005(03)00129-1
M3 - Article
AN - SCOPUS:0038587441
SN - 0925-4005
VL - 92
SP - 199
EP - 207
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-2
ER -