Abstract
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.
Original language | English (US) |
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Pages (from-to) | 1275-1281 |
Number of pages | 7 |
Journal | Science |
Volume | 246 |
Issue number | 4935 |
DOIs | |
State | Published - 1989 |
All Science Journal Classification (ASJC) codes
- General