Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

William D. Huse; Lakshmi Sastry; Sheila A. Iverson; Angray S. Kang; Michelle Alting-Mees; Dennis R. Burton; Stephen J. Benkovic; Richard A. Lerner

doi:10.1126/science.2531466

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

William D. Huse
, Lakshmi Sastry
, Sheila A. Iverson
, Angray S. Kang
, Michelle Alting-Mees
, Dennis R. Burton
, Stephen J. Benkovic
, Richard A. Lerner

Chemistry

Research output: Contribution to journal › Article › peer-review

749 Scopus citations

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

Original language	English (US)
Pages (from-to)	1275-1281
Number of pages	7
Journal	Science
Volume	246
Issue number	4935
DOIs	https://doi.org/10.1126/science.2531466
State	Published - 1989

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title = "Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda",

abstract = "A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.",

author = "Huse, \{William D.\} and Lakshmi Sastry and Iverson, \{Sheila A.\} and Kang, \{Angray S.\} and Michelle Alting-Mees and Burton, \{Dennis R.\} and Benkovic, \{Stephen J.\} and Lerner, \{Richard A.\}",

year = "1989",

doi = "10.1126/science.2531466",

language = "English (US)",

volume = "246",

pages = "1275--1281",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

number = "4935",

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TY - JOUR

T1 - Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

AU - Huse, William D.

AU - Sastry, Lakshmi

AU - Iverson, Sheila A.

AU - Kang, Angray S.

AU - Alting-Mees, Michelle

AU - Burton, Dennis R.

AU - Benkovic, Stephen J.

AU - Lerner, Richard A.

PY - 1989

Y1 - 1989

N2 - A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

AB - A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

UR - https://www.scopus.com/pages/publications/0024833055

UR - https://www.scopus.com/pages/publications/0024833055#tab=citedBy

U2 - 10.1126/science.2531466

DO - 10.1126/science.2531466

M3 - Article

C2 - 2531466

AN - SCOPUS:0024833055

SN - 0036-8075

VL - 246

SP - 1275

EP - 1281

JO - Science

JF - Science

IS - 4935

ER -

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

Abstract

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