Generation of nucleotide-linked resins for identification of novel binding proteins

Shikha S. Chauhan, Emily E. Weinert

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Organisms use numerous nucleotide-containing compounds as intracellular signals to control behavior. Identifying the biomolecules responsible to sensing and responding to changes in signaling molecule concentration is an important area of research. However, identifying the binding proteins can be challenging when there is no prior information available about binding motifs. In this chapter, we describe a straightforward method to generate nucleotide-linked resins for use in pull-down experiments to identify binding proteins. The protocol outlined in this chapter also can be adapted to generate custom resins linked to other molecules of interest.

Original languageEnglish (US)
Title of host publicationIntegrated Methods in Protein Biochemistry
Subtitle of host publicationPart B
EditorsArun K. Shukla
PublisherAcademic Press Inc.
Number of pages8
ISBN (Print)9780323992640
StatePublished - Jan 2023

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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