Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1

Wei Yu; Zhenwu Lin; John P. Hegarty; Xi Chen; Ashley A. Kelly; Yunhua Wang; Lisa S. Poritz; Walter A. Koltun

doi:10.1007/s10753-011-9390-9

Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1

Wei Yu, Zhenwu Lin, John P. Hegarty, Xi Chen, Ashley A. Kelly, Yunhua Wang, Lisa S. Poritz, Walter A. Koltun

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Ulcerative colitis (UC) and Crohn's disease (CD) are two related yet different forms of chronic intestinal inflammation. We investigated the genes regulated by NKX2-3 in B cells from a UC patient by cDNA microarray and compared the results to those genes regulated by NKX2-3 in B cells from a CD patient. Genes regulated by NKX2-3 in B cells from UC were mainly involved in cell growth, inflammation, and immune response. Among the genes regulated by NKX2-3 in both UC and CD, expression of 145 genes was similarly altered and 34 genes was differentially affected by NKX2-3 knockdown. EGR1 was up-regulated in NKX2-3 knockdown B cells from UC while down-regulated in NKX2-3 knockdown B cells from CD. mRNA expressions of NKX2-3 and EGR1 were increased in diseased intestinal tissues from 19 CD patients. NKX2-3 may play different roles in UC and CD pathogenesis by differential regulation of EGR1.

Original language	English (US)
Pages (from-to)	889-899
Number of pages	11
Journal	Inflammation
Volume	35
Issue number	3
DOIs	https://doi.org/10.1007/s10753-011-9390-9
State	Published - Jun 2012

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

Access to Document

10.1007/s10753-011-9390-9

Cite this

@article{2d5abf4619674f01b6d7afc4dd79dacb,

title = "Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1",

abstract = "Ulcerative colitis (UC) and Crohn's disease (CD) are two related yet different forms of chronic intestinal inflammation. We investigated the genes regulated by NKX2-3 in B cells from a UC patient by cDNA microarray and compared the results to those genes regulated by NKX2-3 in B cells from a CD patient. Genes regulated by NKX2-3 in B cells from UC were mainly involved in cell growth, inflammation, and immune response. Among the genes regulated by NKX2-3 in both UC and CD, expression of 145 genes was similarly altered and 34 genes was differentially affected by NKX2-3 knockdown. EGR1 was up-regulated in NKX2-3 knockdown B cells from UC while down-regulated in NKX2-3 knockdown B cells from CD. mRNA expressions of NKX2-3 and EGR1 were increased in diseased intestinal tissues from 19 CD patients. NKX2-3 may play different roles in UC and CD pathogenesis by differential regulation of EGR1.",

author = "Wei Yu and Zhenwu Lin and Hegarty, {John P.} and Xi Chen and Kelly, {Ashley A.} and Yunhua Wang and Poritz, {Lisa S.} and Koltun, {Walter A.}",

note = "Funding Information: This work was supported by a grant from the Philadelphia Health Care Trust (WAK), the Carlino fund for IBD research at the Milton S. Hershey Medical Center (WAK), Penn State College of Medicine, and the Milton S. Hershey Penn State College of Medicine Surgery Research Feasibility Grant (WY).",

year = "2012",

month = jun,

doi = "10.1007/s10753-011-9390-9",

language = "English (US)",

volume = "35",

pages = "889--899",

journal = "Inflammation",

issn = "0360-3997",

publisher = "Springer New York",

number = "3",

}

TY - JOUR

T1 - Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1

AU - Yu, Wei

AU - Lin, Zhenwu

AU - Hegarty, John P.

AU - Chen, Xi

AU - Kelly, Ashley A.

AU - Wang, Yunhua

AU - Poritz, Lisa S.

AU - Koltun, Walter A.

N1 - Funding Information: This work was supported by a grant from the Philadelphia Health Care Trust (WAK), the Carlino fund for IBD research at the Milton S. Hershey Medical Center (WAK), Penn State College of Medicine, and the Milton S. Hershey Penn State College of Medicine Surgery Research Feasibility Grant (WY).

PY - 2012/6

Y1 - 2012/6

N2 - Ulcerative colitis (UC) and Crohn's disease (CD) are two related yet different forms of chronic intestinal inflammation. We investigated the genes regulated by NKX2-3 in B cells from a UC patient by cDNA microarray and compared the results to those genes regulated by NKX2-3 in B cells from a CD patient. Genes regulated by NKX2-3 in B cells from UC were mainly involved in cell growth, inflammation, and immune response. Among the genes regulated by NKX2-3 in both UC and CD, expression of 145 genes was similarly altered and 34 genes was differentially affected by NKX2-3 knockdown. EGR1 was up-regulated in NKX2-3 knockdown B cells from UC while down-regulated in NKX2-3 knockdown B cells from CD. mRNA expressions of NKX2-3 and EGR1 were increased in diseased intestinal tissues from 19 CD patients. NKX2-3 may play different roles in UC and CD pathogenesis by differential regulation of EGR1.

AB - Ulcerative colitis (UC) and Crohn's disease (CD) are two related yet different forms of chronic intestinal inflammation. We investigated the genes regulated by NKX2-3 in B cells from a UC patient by cDNA microarray and compared the results to those genes regulated by NKX2-3 in B cells from a CD patient. Genes regulated by NKX2-3 in B cells from UC were mainly involved in cell growth, inflammation, and immune response. Among the genes regulated by NKX2-3 in both UC and CD, expression of 145 genes was similarly altered and 34 genes was differentially affected by NKX2-3 knockdown. EGR1 was up-regulated in NKX2-3 knockdown B cells from UC while down-regulated in NKX2-3 knockdown B cells from CD. mRNA expressions of NKX2-3 and EGR1 were increased in diseased intestinal tissues from 19 CD patients. NKX2-3 may play different roles in UC and CD pathogenesis by differential regulation of EGR1.

UR - http://www.scopus.com/inward/record.url?scp=84863798199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863798199&partnerID=8YFLogxK

U2 - 10.1007/s10753-011-9390-9

DO - 10.1007/s10753-011-9390-9

M3 - Article

C2 - 21968973

AN - SCOPUS:84863798199

SN - 0360-3997

VL - 35

SP - 889

EP - 899

JO - Inflammation

JF - Inflammation

IS - 3

ER -

Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this