Genetic analysis of CRPV pathogenesis: The L1 open reading frame is dispensable for cellular transformation but is required for papilloma formation

M. Nasseri; C. Meyers; F. O. Wettstein

doi:10.1016/0042-6822(89)90388-7

Genetic analysis of CRPV pathogenesis: The L1 open reading frame is dispensable for cellular transformation but is required for papilloma formation

M. Nasseri, C. Meyers, F. O. Wettstein

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Abstract

Genetic studies to elucidate the role of papillomaviruses in the development and progression of tumors have been severely hampered because the viruses cannot be grown in tissue culture and therefore mutants are not available. We have employed recombinant DNA for papilloma induction to identify essential sequences involved in papillomavirus pathogenesis. Here, we demonstrated that deleting most of the open reading frame (ORF)L2 did not affect the potential of viral cottontail rabbit papillomavirus (CRPV) DNA to induce papillomas. The extrachromosomally maintained DNA in the papillomas was not rearranged and the major transcripts of 1.3 and 2.0 kb encoded E7 and E6, respectively. A recombinant DNA containing a larger deletion lacking the 3′ terminal half of ORF L2 and all of ORF L1 (pdlBcll) did not induce papillomas. The results indicate that sequences in the late region not required for transformation of NIH 3T3 cells by bovine papillomavirus type-1 essential in CRPV for induction of papillomas.

Original language	English (US)
Pages (from-to)	321-325
Number of pages	5
Journal	Virology
Volume	170
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(89)90388-7
State	Published - May 1989

All Science Journal Classification (ASJC) codes

Virology

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title = "Genetic analysis of CRPV pathogenesis: The L1 open reading frame is dispensable for cellular transformation but is required for papilloma formation",

abstract = "Genetic studies to elucidate the role of papillomaviruses in the development and progression of tumors have been severely hampered because the viruses cannot be grown in tissue culture and therefore mutants are not available. We have employed recombinant DNA for papilloma induction to identify essential sequences involved in papillomavirus pathogenesis. Here, we demonstrated that deleting most of the open reading frame (ORF)L2 did not affect the potential of viral cottontail rabbit papillomavirus (CRPV) DNA to induce papillomas. The extrachromosomally maintained DNA in the papillomas was not rearranged and the major transcripts of 1.3 and 2.0 kb encoded E7 and E6, respectively. A recombinant DNA containing a larger deletion lacking the 3′ terminal half of ORF L2 and all of ORF L1 (pdlBcll) did not induce papillomas. The results indicate that sequences in the late region not required for transformation of NIH 3T3 cells by bovine papillomavirus type-1 essential in CRPV for induction of papillomas.",

author = "M. Nasseri and C. Meyers and Wettstein, {F. O.}",

note = "Funding Information: We thank Margerita Tayag for excellent technical help and Jeannie Martinez and Lori Kenton for help with the manuscript. M.N. was the recipient of a postdoctoral fellowship from the Cancer Research Coordinating Committee of the University of California and CM. was a recipient of a predoctoral fellowship from U.S. Public Health Service Grants GM 07104 and Al 07323. The research was supported by Grant CA 1815 1 awarded by the National Cancer Institute, National Institutes for Health, Department of Health and Human Services, and a grant from the W.M. Keck Foundation.",

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N1 - Funding Information: We thank Margerita Tayag for excellent technical help and Jeannie Martinez and Lori Kenton for help with the manuscript. M.N. was the recipient of a postdoctoral fellowship from the Cancer Research Coordinating Committee of the University of California and CM. was a recipient of a predoctoral fellowship from U.S. Public Health Service Grants GM 07104 and Al 07323. The research was supported by Grant CA 1815 1 awarded by the National Cancer Institute, National Institutes for Health, Department of Health and Human Services, and a grant from the W.M. Keck Foundation.

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N2 - Genetic studies to elucidate the role of papillomaviruses in the development and progression of tumors have been severely hampered because the viruses cannot be grown in tissue culture and therefore mutants are not available. We have employed recombinant DNA for papilloma induction to identify essential sequences involved in papillomavirus pathogenesis. Here, we demonstrated that deleting most of the open reading frame (ORF)L2 did not affect the potential of viral cottontail rabbit papillomavirus (CRPV) DNA to induce papillomas. The extrachromosomally maintained DNA in the papillomas was not rearranged and the major transcripts of 1.3 and 2.0 kb encoded E7 and E6, respectively. A recombinant DNA containing a larger deletion lacking the 3′ terminal half of ORF L2 and all of ORF L1 (pdlBcll) did not induce papillomas. The results indicate that sequences in the late region not required for transformation of NIH 3T3 cells by bovine papillomavirus type-1 essential in CRPV for induction of papillomas.

AB - Genetic studies to elucidate the role of papillomaviruses in the development and progression of tumors have been severely hampered because the viruses cannot be grown in tissue culture and therefore mutants are not available. We have employed recombinant DNA for papilloma induction to identify essential sequences involved in papillomavirus pathogenesis. Here, we demonstrated that deleting most of the open reading frame (ORF)L2 did not affect the potential of viral cottontail rabbit papillomavirus (CRPV) DNA to induce papillomas. The extrachromosomally maintained DNA in the papillomas was not rearranged and the major transcripts of 1.3 and 2.0 kb encoded E7 and E6, respectively. A recombinant DNA containing a larger deletion lacking the 3′ terminal half of ORF L2 and all of ORF L1 (pdlBcll) did not induce papillomas. The results indicate that sequences in the late region not required for transformation of NIH 3T3 cells by bovine papillomavirus type-1 essential in CRPV for induction of papillomas.

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Genetic analysis of CRPV pathogenesis: The L1 open reading frame is dispensable for cellular transformation but is required for papilloma formation

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