@article{5a7268ebf55d414180dc8b0103f2b658,
title = "Genetic analysis of the RNA polymerase II CTD in Drosophila",
abstract = "The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.",
author = "Feiyue Lu and Gilmour, {David S.}",
note = "Funding Information: We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing the transgenic Rpb1 RNAi fly stock used in this study. This work was funded by NIH/NIGMS R01-GM047477 (to D.S.G.). Illustrations of genetic mating schemes in all figures were generated using Genotype Builder [31]. Funding Information: We thank the TRiP at Harvard Medical School ( NIH / NIGMS R01-GM084947 ) for providing the transgenic Rpb1 RNAi fly stock used in this study. This work was funded by NIH/NIGMS R01-GM047477 (to D.S.G.). Illustrations of genetic mating schemes in all figures were generated using Genotype Builder [31] . Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = apr,
day = "15",
doi = "10.1016/j.ymeth.2019.01.010",
language = "English (US)",
volume = "159-160",
pages = "129--137",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press Inc.",
}