Genetic analysis of the RNA polymerase II CTD in Drosophila

Feiyue Lu; David S. Gilmour

doi:10.1016/j.ymeth.2019.01.010

Genetic analysis of the RNA polymerase II CTD in Drosophila

Feiyue Lu
, David S. Gilmour

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.

Original language	English (US)
Pages (from-to)	129-137
Number of pages	9
Journal	Methods
Volume	159-160
DOIs	https://doi.org/10.1016/j.ymeth.2019.01.010
State	Published - Apr 15 2019

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2019.01.010

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title = "Genetic analysis of the RNA polymerase II CTD in Drosophila",

abstract = "The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.",

author = "Feiyue Lu and Gilmour, \{David S.\}",

note = "Funding Information: We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing the transgenic Rpb1 RNAi fly stock used in this study. This work was funded by NIH/NIGMS R01-GM047477 (to D.S.G.). Illustrations of genetic mating schemes in all figures were generated using Genotype Builder [31]. Funding Information: We thank the TRiP at Harvard Medical School ( NIH / NIGMS R01-GM084947 ) for providing the transgenic Rpb1 RNAi fly stock used in this study. This work was funded by NIH/NIGMS R01-GM047477 (to D.S.G.). Illustrations of genetic mating schemes in all figures were generated using Genotype Builder [31] . Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

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day = "15",

doi = "10.1016/j.ymeth.2019.01.010",

language = "English (US)",

volume = "159-160",

pages = "129--137",

journal = "Methods",

issn = "1046-2023",

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T1 - Genetic analysis of the RNA polymerase II CTD in Drosophila

AU - Lu, Feiyue

AU - Gilmour, David S.

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PY - 2019/4/15

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N2 - The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.

AB - The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.

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UR - https://www.scopus.com/pages/publications/85060925034#tab=citedBy

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DO - 10.1016/j.ymeth.2019.01.010

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SN - 1046-2023

VL - 159-160

SP - 129

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JO - Methods

JF - Methods

ER -

Genetic analysis of the RNA polymerase II CTD in Drosophila

Abstract

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